Diaminobenzidine
Mostrando 1-12 de 69 artigos, teses e dissertações.
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1. Synthesis of Poly(Vinylferrocene) Perchlorate/Poly(3,3’-Diaminobenzidine) Modified Electrode in Dichloromethane for Electroanalysis of Hydroquinone
Poly(vinylferrocenium) perchlorate-poly(3,3’-diaminobenzidine) (PVF+ClO4–-PDAB) composite film was synthesized electrochemically for the first time on Pt disc electrode in a dichloromethane solution containing poly(vinylferrocene) (PVF) polymer, 3,3’-diaminobenzidine (DAB) monomer and tetrabutylammonium perchlorate as supporting electrolyte. This modif
J. Braz. Chem. Soc.. Publicado em: 2015-09
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2. Desenvolvimento e padronização do Dot-ELISA usando peptídeos recombinantes para o diagnóstico sorológico de Neospora caninum
A neosporose é reconhecida como uma das maiores causas de aborto e perdas neonatais em bovinos de leite e corte em todo o mundo. Nos últimos anos esta doença tem atraído o interesse de pesquisadores com foco na epidemiologia e métodos eficazes de diagnóstico desta doença. No presente estudo objetivou-se desenvolver e padronizar um teste Dot-ELISA para
Pesq. Vet. Bras.. Publicado em: 2014-08
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3. Efeito do tratamento com antioxidantes na qualidade de espermatozóides criopreservados de cães / Effect of antioxidants on post-thaw canine sperm quality
One of the main factors known to impair post thaw semen quality is oxidative stress (i.e., higher production of reactive oxygen species not compensated by improved antioxidant protection). The present experiment aimed to evaluate the effects of antioxidant treatment with glutathione (GSH) and vitamin E on the quality of dogs cryopreserved semen. Towards this
Publicado em: 2009
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4. Carcinoma ductal invasivo mamÃrio esporÃdico: uma abordagem histoquÃmica e imunohistiquÃmica
Breast cancer is the most frequent neoplasia and the main cause of cancer death in women worldwide. Among the several types of mammary gland neoplasia Invasive Ductal Carcinoma (IDC) represents 65% to 80% of all carcinomas. The major problem concerning this neoplasia is the great genotypic and phenotypic heterogeneity. This feature explains the increasing se
Publicado em: 2009
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5. MAPEAMENTO FÍSICO DE GENES EXPRESSOS DE Anopheles darlingi ROOT, 1926 E SUA ANÁLISE in silico EM Anopheles gambiae GILES, 1902 (DIPTERA: CULICIDAE)
Na região Amazônica brasileira ocorrem cerca de 99,7% dos casos de malária do Brasil, onde o principal vetor é o Anopheles darlingi. Em 2007, na região da Amazônia Legal, foram relatados aproximadamente 185 mil casos da doença. Há a necessidade de estudos para o entendimento do genoma funcional do A. darlingi, considerando a pouca quantidade de sequ�
Publicado em: 2009
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6. ImunohistoquÃmica e anÃlise digital de imagens no carcinoma ductal infiltrante
The immunohistochemistry is a technique of great help in diagnosing diseases of the breast, including tumors. Equally important, the analysis of digital images is being increasingly used in studies of changes in the breast. This study aimed to quantify the expression of the antibody morfometricamente through immunoperoxidase as in normal breast tissue, and i
Publicado em: 2008
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7. ExpressÃo tecidual da proteÃna cerbB-2 em mulheres portadoras de doenÃas tumorais de mama
The cerbB-2 protein has been sufficiently studied for its importance as prognostic factor of the breast cancer, mainly in the chemotherapy associations. To eliminate the subjectivity in the interpretation of the conventional immunohistichemistry method, this study aims to quantify, morphometrically, the immunostain of the cerbB-2 protein expressed in breast
Publicado em: 2007
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8. Colheita, análise e criopreservação de sêmen de uma espécie modelo de primata neotropical, Sagui-de-Tufo-Branco (Callithrix jacchus) / Collection, analysis and cryopreservation of semen from a model species, the common marmoset (Callithrix jacchus)
The Callithrix jacchus can be used as a model species for other Neotropical primates species, mainly Callithriquids, for reproductive studies. Semen samples were collected with the following objectives: to improve the penile vibrostimulation technique, validate sperm evaluation techniques for field work, analyse the semen characteristics from animals at 2 co
Publicado em: 2007
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9. Padronização da técnica de imunoistoquímica para o diagnóstico etiológico de rotina da diarréia bovina a vírus
The immunohistochemistry standardization for bovine viral diarrhea virus (BVDV) diagnostic was described. The formalin-fixed tissue samples from a heifer with mucosal disease were used as positive control. The validation of the first phase results was performed using samples from an aborted fetus and a calf infected with reference strains of BVDV. The best r
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Publicado em: 2005-10
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10. Density, proportion, and dendritic coverage of retinal ganglion cells of the common marmoset (Callithrix jacchus jacchus)
We performed a quantitative analysis of M and P cell mosaics of the common-marmoset retina. Ganglion cells were labeled retrogradely from optic nerve deposits of Biocytin. The labeling was visualized using horseradish peroxidase (HRP) histochemistry and 3-3'diaminobenzidine as chromogen. M and P cells were morphologically similar to those found in Old- and N
Brazilian Journal of Medical and Biological Research. Publicado em: 2005-06
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11. Does the correlation between EBNA-1 and p63 expression in breast carcinomas provide a clue to tumorigenesis in Epstein-Barr virus-related breast malignancies?
Several investigators have identified Epstein-Barr virus (EBV) particles in breast carcinomas, a fact that supports a role for EBV in mammary tumorigenesis. The possible mechanism involved in this process is not clear. The present study was carried out in an attempt to determine whether there is a relationship between latent infection with EBV and p53 and p6
Brazilian Journal of Medical and Biological Research. Publicado em: 2004-01
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12. Construction of a mutant library of horseradish peroxidase gene by directed evolution and development of an in situ screening method
A process of directed evolution applied to obtain a library of mutants of horseradish peroxidase (HRP) enzyme is described. We have introduced slight variations into the original DNA shuffling protocol. A DNA template was prepared by PCR amplification and digested with Dnase I during 1 hour. Dnase I products were concentrated by precipitation with isopropano
Brazilian Journal of Chemical Engineering. Publicado em: 2003-03