Carcinoma ductal invasivo mamÃrio esporÃdico: uma abordagem histoquÃmica e imunohistiquÃmica

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

Breast cancer is the most frequent neoplasia and the main cause of cancer death in women worldwide. Among the several types of mammary gland neoplasia Invasive Ductal Carcinoma (IDC) represents 65% to 80% of all carcinomas. The major problem concerning this neoplasia is the great genotypic and phenotypic heterogeneity. This feature explains the increasing search for biomarkers of breast cancer in recent years. Among these probes lectins, (glycol)protein that recognizes carbohydrates, have been used as decipherers of cell glycocodes and biomarkers in cancer. This study aimed to evaluate the carbohydrate profile in IDC by lectin histochemistry and to correlate it to clinical and histopathological parameters such as age, tumor size, histological variant and p53 expression. Eighty-eight biopsies of IDC, and 20 biopsies of reductional mastoplasty were obtained at the Clinic Hospital of the Federal University of the State of Pernambuco. Sixty-eight ICD free borders were used as normal controls besides the reductional mastoplasty. For lectin histochemistry tissue sections (4μm) were treated with trypsin (0.1%), methanol-hydrogen peroxide (0.3%) and than incubated with horseradish peroxidase (HRP) conjugated lectins (Concanavalin A, Con A-HRP, Ulex Europeans I, UEA-I-HRP, Peanult Aglutinin, PNA-HRP; specific for glucose/mannose, D-galactose and L-fucose respectively. p53 immunohistochemistry was performed using the estreptavidin-biotin-peroxidase technique. Both reactions were revealed with diaminobenzidine (DAB)-H2O2, counter-stained with haematoxylin and evaluated on optical micrsocopy. There is a significant association between lectins Con A (p<0.001), PNA (p<0.001) and UEA-I (p<0.001) in the two study groups, normal and IDC. The prevalence of positivity was significantly higher in the group of cancer. Mutant protein of p53 was detected in 34,1% of CDI biopsies. Morphologically PNA recognized atypical mitosis and Con A and UEA-I vascular endothelium. All lectins of this study recognize neoplastics cells. There is no association with lectins and clinical parameters (p>0.005). Results indicated that there is a higher disponibility of glucose/mannose, L-fucose and Dgalactose residues in IDC when compared to normal tissue indicating these lectins as biomarkers for this malignancy

ASSUNTO(S)

histoquÃmica com lectinas carcinoma ductal invasivo p53 bioquimica p53 lectin histochemistry invasive ductal carcinoma

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