Diaminobenzidine
Mostrando 13-24 de 69 artigos, teses e dissertações.
-
13. Cytochemical Localization of Catalase in Glyoxysomes Isolated from Maize Scutella
The localization of catalase in isolated maize scutellum glyoxysomes was investigated by means of the diaminobenzidine histochemical reaction. Only the membranes of the glyoxysomes become heavily stained after incubation with diaminobenzidine and H2O2. If the glyoxysomes are lysed with Tricine buffer at pH 9, 70% of the catalase is solubilized, while the rem
-
14. Cytochemical Localization of Catalase Activity in Glyoxysomes from Castor Bean Endosperm 1
Glyoxysomes isolated from castor bean (Ricinus communis L. var. zanzibariensis) endosperm have been stained by the cytochemical diaminobenzidine reaction. The reaction product obtained by preincubation with 3,3′-diaminobenzidine and incubation with the reagent and H2O2 is distributed uniformly throughout the matrix of the organelles. Ricinosomes or dilated
-
15. Effects of Chloramphenicol on the Mitochondrial Respiratory Chain in the Wild Strain and in a Cytoplasmic Chloramphenicol-Resistant Mutant of Tetrahymena pyriformis
The effects of chloramphenicol (CAP) on mitochondrial respiratory activity in the wild strain (ST) and in a cytoplasmic CAP-resistant mutant (STR1) of Tetrahymena pyriformis were studied by determining oxygen consumption, by spectrophotometry, and by cytochemistry. In the absence of CAP both strains had the same respiration capacity, and the low-temperature
-
16. Ultrastructural localization of Thermoplasma acidophilum surface carbohydrate by using concanavalin A.
Surface carbohydrate, presumably the lipopolysaccharide, of Thermoplasma acidophilum was visualized by means of the concanavalin A, horseradish peroxidase, and diaminobenzidine cytochemical staining procedure.
-
17. Ultrastructural Visualization of Surface Carbohydrate Structures on Mycoplasma Membranes by Concanavalin A
Surface carbohydrates of Mycoplasma mycoides var. capri were made visible by the cytochemical staining procedure with concanavalin A, horseradish peroxidase, and diaminobenzidine.
-
18. Cytochemical Localization of Catalase Activity in Yeast Peroxisomes
Diaminobenzidine oxidation product occurred in peroxisomes and in the intracristate spaces of mitochondria. The reaction was inhibited only in peroxisomes when 3-amino-1,2,4-triazole was present, but cyanide and azide inhibited deposition in both kinds of organelles.
-
19. Silver enhancement of polymerised diaminobenzidine: increased sensitivity for immunoperoxidase staining.
Unambiguous identification of lymphocytes is sometimes difficult because of weak immunostaining of the cell membrane immunoglobulins. A simple method of intensifying the diaminobenzidine (DAB) peroxidase reaction was therefore devised. Paraffin wax sections of formalin fixed tonsils and lymphomas were digested with trypsin and immunostained for kappa and lam
-
20. The Effect of Water Stress on Mitochondria of Root Cells: A Biochemical and Cytochemical Study 1
Water loss from root cells of Zea mays induced by exposure to severe water stress, caused a reduction in the respiratory rate (O2 consumption) and at the same time an increase in activity of the mitochondrial cytochrome oxidase (histochemical oxidation of diaminobenzidine). The connections between these changes and the changes in the fine structure of the mi
-
21. Identification of the Endodermal Vacuole as the Iron Storage Compartment in the Arabidopsis Embryo1
Deciphering how cellular iron (Fe) pools are formed, where they are localized, and which ones are remobilized represents an important challenge to better understand Fe homeostasis. The recent development of imaging techniques, adapted to plants, has helped gain insight into these events. We have analyzed the localization of Fe during embryo development in Ar
American Society of Plant Biologists.
-
22. The Mycorrhizal Fungus Gigaspora margarita Possesses a CuZn Superoxide Dismutase That Is Up-Regulated during Symbiosis with Legume Hosts1
A full-length cDNA showing high similarity to previously described CuZn superoxide dismutases (SODs) was identified in an expressed sequence tag collection from germinated spores of the arbuscular mycorrhizal fungus Gigaspora margarita (BEG 34). The corresponding gene sequence, named GmarCuZnSOD, is composed of four exons. As revealed by heterologous complem
American Society of Plant Biologists.
-
23. Immunocytochemical localization of gamma-glutamyltransferase in rat kidney with protein A-horseradish peroxidase.
Immunocytochemistry with a specific antiserum and protein A-horseradish peroxidase permits visualization of the sites of gamma-glutamyltransferase [(5-glutamyl)-peptide:amino-acid 5-glutamyltransferase, EC 2.3.2.2] at both the light- and the electron-microscope levels. As seen by light microscopy, the enzyme is localized only in the proximal convolutions of
-
24. Ultrastructural visualisation of carbohydrate groups in the surface coating of hamster alveolar macrophages and pneumonocytes.
The surface coating of the alveolar macrophages and pneumonocytes of hamster lung was studied using an electron microscopy technique. Slices of lung tissue were fixed in aldehyde, labelled with a battery of lectin-horseradish peroxidase conjugates, incubated in a diaminobenzidine-hydrogen peroxide medium and then postfixed in an osmium tetroxide solution. Th