Purificação da alfa-Lactalbumina a partir do soro de leite em leito fixo e expandido de resinas

AUTOR(ES)

Vinicius Veredas

DATA DE PUBLICAÇÃO

2000

RESUMO

The interest and applications of biotechnology products has been increasing the development of new recovery and purification processes for proteins. The bovine milk serum, obtained from casein manufacture for cheese production, is mostly rejected into watercourse, causing problems to the environment due to its high biological oxygen demand (BOD). The proteins of milk serum have excellent nutritious and pharmaceutical value, h oweve r, its application for protein enrichment of food products is limited due to its low content in the milk serum. The main proteins of milk serum are: ~-Iactoglobulins, a-Iactalbumine, bovine serum albumine, immunoglobulins, lactoperoxidase, lactoferrin, lisozime and other lower content proteins which have a high aggregate value. The a-Iactalbumine acts in the human organism by stimulating the agents of the immunologycal system due to increasing on glutathione levei in several organs and blood, resulting in benefits for patients of some diseases like Parkinson and Alzheimer s iII, cancer and AIDS. It was studied in this work the separation processes of a-Iactalbumine, by chromatographic techniques making use of fixed and expanded bed methods. The expanded bed enables cost reduction on purification process by reducing separation steps used for removing suspended solids, as in case of milk serum extracts. In our experiments were studied the adsorption conditions of alactalbumine aiming at its purification by using ionic exchange and hydrophobic interaction adsorbents. Other experiments were accomplished at stirred tank systems for the determination of isotherms and adsorption kinetics. It was obtained, in this work, an a-Iactalbumine purity higher than 80%, with a five fold purification factor by using the hydrophobic interaction resins in a single purification step

ASSUNTO(S)

adsorção adsorption alfa-lactalbumine ionic exchange analise cromatografica milk serum proteinas - purificação hydrophobic interaction soro do leite

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