Produção de glutaminase por Zymomonas mobilis cp4 na fermentação de glicose utilizando ferramentas estatísticas

AUTOR(ES)

Gilcelene Bruzon

DATA DE PUBLICAÇÃO

2006

RESUMO

The enzyme glutaminase is used in the industry as flavor-enhancing and for the nutritional enrichment of food. Besides, its pharmaceutical potential application could be used in both theanina production and as anti-leukemic agent. The bacterium Zymomonas mobilis is found in regions of tropical climate and it has been studied for ethanol production. Conditions of cultivation (stillness, shaking and fermentation time) and medium composition (presence and absence of component) for glutaminase production by Zymomonas mobilis CP4 using statistical analysis have been carried out. Variance analysis in blocks and test of Tukey showed that components of the medium of cultivation, stillness and time of fermentation were important. In the minimum medium optimization was used glucose (10, 30, and 50g/L), glutamine (0, 0,5 and 1g/L) and time of fermentation (18, 24 and 30 hours). A maximum production of 8,86 U/L of glutaminase was obtained. Glutaminase production Kinetics by Zymomonas mobilis CP4 under optimized conditions showed a typically like-exponential growth. The glutaminase production showed to be related with the biomass production.

ASSUNTO(S)

fermentation glutamine enzimas microbianas - análise zymomonas mobilis fermentação microbiologia industrial glutaminase industrial microbiology

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