Estudo comparativo da fermentação de glicose e sacarose por Zymomenas mobilis

AUTOR(ES)
DATA DE PUBLICAÇÃO

1985

RESUMO

Z.mobilis NRRL B-806 produced more sucrose hydrolyzing enzyme (S.H.E.) on me di um containing fructose than other tested strains. This extracelular enzyme hydrolysed sucrose and raffinose, but did not hydrolyse melezitose resembling a S-D-fructofuranoside fructohydrolase (E.C. 3.2.1.26). Z.mobilis NRRL B-14022 is able to grow ln a medium containing sucrose and produces the sucrose hidro1ysing enzyme. This strain rapid1y utilized sucrose to ethanol as compared to other tested strains. Therefore this strain was used for comparison of fermentation of glucose and sucrose. Comparative fermentation of glucose and sucrose was studied by fermentating respective glucose and sucrose concentration 100~200 g/L at 30 and 35°C by this microorganism. Kinetic parameters u, qp , Yp/s, e Yx/s were estimated. The demonstrated that yields of ethanol from glucose were results higher than sucrose fermentation. Highest ethanol yield of glucose was obtained when concentration of substrate 200 g/L was fermented at 30°C. In the case of sucrose fermentation, highest yield was obtained when sucrose concentration 100 g/L was fermented at 35°C. Values of maximum specific growth rate, μmax , for glucose max, fermentations were lower than sucrose fermentations. Sucrose fermentation by this microorganism, produced 1evan at 30°C, whereas formation of levan was inhibited at 35ºC. Concentrations of residual sugars after fermentation of sucrose at 30oC, were higher than when glucose was used. In the case of fermentation of sucrose at 35°C, the concentrations of residual sugars were slightly decreased as compared with 30°C. In fermentation of glucose at 35°C the concentrations of residual sugars were increased markedly in comparison with 30°C.

ASSUNTO(S)

fermentação sacarose glicose

ACESSO AO ARTIGO

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