CaracterizaÃÃo BioquÃmica, FisiolÃgica e Ultraestrutural do Processo de BiossorÃÃo do Cobre por Cunninghamella elegans - UCP 542 / CaracterizaÃÃo BioquÃmica, FisiolÃgica e Ultraestrutural do Processo de BiossorÃÃo do Cobre por Cunninghamella elegans - UCP 542

AUTOR(ES)
DATA DE PUBLICAÇÃO

2004

RESUMO

The aim of this work was to evaluate the physiological, biochemical and ultrastructural aspects of C. elegans grown in copper presence. The growth profile was obtained by using biomass yields, glucose and phosphate consumption and pH. The polyphosphate profile, phosphatases activities and metal remotion were also studied. The results revealed the copper effects on the isolate growth according to the biomass yields. Glucose and phosphate consumption was similar to the control and treated cultures. The copper presence in the culture media induced variations on the polyphosphate profile. The polyphosphate level was almost constant in the beginning of control culture growth, and could be related to the exponential growth phase. On the other hand, the treated cultures exhibited a significant reduction in the polyphosphate levels, indicating an active metabolization of the polymer. The acid phosphatase activity was not detected in the isolate studied. However, the alkaline phosphatase activity was significantly lower in the treated cultures. By using the atomic absortion spectrophotometry the copper remotion of culture medium was observed during the microorganism growth. The ultrastructural analysis revealed differences in the C. elegans mycelia grown in copper presence. Variations related to the cell electrondensity, chlamidospores form and number, hyphal ramification process, hyphal texture and form were detected in the samples submitted to the metal treatment. The results revealed the potential use of C. elegans isolate in the remediation and biosorption studies in environments polluted by copper

ASSUNTO(S)

cobre fosfatases polifosfato cunninghamella elegans micologia cunninghamella elegans polyphosphate copper phosphatases

ACESSO AO ARTIGO

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