Morfotipagem, caracterizaÃÃo fisiolÃgica de leveduras isoladas de secreÃÃo vaginal e sensibilidade a prÃpolis e lectinas

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

The yeasts, especially the candidoses, are assuming a role of great importance in the human pathology. The female genital tract may be affected by infectious and inflammatory processes, which are diagnosed by examination of vaginal secretion. Several species of yeasts have been isolated from the vulva and vagina in normal cases and vulvovaginitis. Aiming to characterize physiologically, to perform morphotyping, examine the enzymatic profile of these fungi and assess antifungal activity of Brazilian propolis (green and red), as well as lectins from seeds of leguminous plants in Brazil (Dviol, DRL, ConBr and LSL), thirty samples from twenty-three yeasts species isolated from vaginal secretion of pregnant and non pregnant women were used. All cultures are deposited in mycology collection - URM, Department of Mycology, Federal University of Pernambuco and were subjected to taxonomic confirmation according to the traditional criteria which are based on morphological and physiological evidence. The morphotyping was used as a method of differentiation between species. It was found that from all the 30 isolates, all of them (100%) presented growth at room temperature (RT = 28 Â C Â 1 Â C) and at 37ÂC, while 17 isolates (57%) grew at 42ÂC. The morphotypes showed significant differences regardless of species, symptomatic presence or absence. Concerning the detection of enzyme activity, proteasic activity was observed in two isolates (6Â6%) Candida maritima URM 4976 and C. obtusa URM 4982. The phospholipasic activity was detected in 20 isolates (66,7%), using the soybean lecithin as substrate at different temperatures. From the 30 yeasts samples tested, all were sensitive to amphotericin B with CIMs ranging from 0,06 ug/mL to 1 ug/mL. In relation to fluconazole, six samples showed resistance with CIMs higher than 64 ug/mL. From the yeast samples analyzed, only three isolates were susceptible to the aqueous extract of the green propolis. The alcoholic extract of the green propolis showed antifungal activity between 2-1,024μg/mL. The fungicidal activity of the alcoholic extract of the red propolis was found in all samples, the geometric mean was between 8 â 161,1 g/mL. The data show that all yeasts samples isolated from vaginal secretion was sensitive to the alcohol extract of the red propolis. The antifungal potential of red propolis for these species suggest future applications as an alternative treatment for vaginal yeasts. The other lectins DRL, ConBr and DvioL, showed antifungal potential against yeast isolated from vaginal secretion. These findings offering offer a promising field of investigation to develop new therapeutic strategies against vaginal yeast infections, collaborating to improve womenâs health. Thus, the characterization of yeasts isolated from vaginal secretion, and the determination of thier enzymatic activity may contribute to understanding the epidemiology of vulvovaginitis and assist in the treatment of patients

ASSUNTO(S)

patogenicidade prÃpolis propolis leveduras yeasts micologia lectinas morphotyping pathogenicity lectins morfotipagem leveduroses

ACESSO AO ARTIGO

Documentos Relacionados