Aspectos bioquÃmicos e fisiolÃgicos do acÃmulo de polifosfato inorgÃnico por Cunninghamella elegans

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

Inorganic polyphosphate (PolyP) is a homopolymer of phosphate linked by phosphoanhydride bonds, first indentified a hundred years ago. This molecule is ubiquitous in nature, suggesting the PolyP envolvement in important metabolics process. The fungi are a very important group of organism showing a large application for various biotechnology areas. Despite of the great importace of PolyP, they metabolic role of production, as well as, they functions are still unclear, mainly in eucariotcs organisms. In this work was investigated the biochemistry and physiological aspects of PolyP accumulation by ten Cunninghamella elegans strains. The experiments were carried out acoording to a two-level factorial design (22), without central point. The results obtained showed the significative influence of glucose concentration in the culture medium and temperature on the growth and PolyP accumulation. The factorial arrangement alowed the construction of mathematics models for PolyP acumulation for each tested strain. C. elegans UCP 542 showed the highest yield of 5.77 μg de Pi/mg of dried biomass. The PolyP accumulation by C. elegans occurs during all phases of growth. The alkali-soluble is the mainly fraction produced. Activities enzymatic of phosphatases acid and alkaline were detected for all strains, however donât show correlation with PolyP accumulation. All strains did not show ability for PolyP accumulation, mainly C. elegans 542 that showed excellent yield, showing a great potential for application in biotechnological process

ASSUNTO(S)

fisiologia bioquÃmica cunninghamella elegans micologia polifosfato

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