Aspectos bioquÃmicos e citoquÃmicos do polifosfato em Cunninghamella elegans

AUTOR(ES)
DATA DE PUBLICAÇÃO

2003

RESUMO

The growth profile, phosphate and glucose uptake, phosphorus content, and polyphosphate distribution, structure and location were evaluated in cunninghamella elegans grown in media containing different phosphate concetrations. The results showed a signinificant influence of the concentration of phosphate on biomass production and polyphosphate metabolism. The highest phosphate concentration induced the best yields of biomass. A correlation among phosphate and glucose uptake, growth and total polyphosphate content in the mycelia and the medium phosphate concentration was observed. Cellular fractions of Polyphosphate were identified and determined by using sequential extraction procedures. The ultrastructural cytochemistry was used successfully to analyze the polyphosphate location and distribution in C. elegans. The results revealed differences on the pattern of reaction products deposition in the mycelia according to the cellular growth phase and phosphate concentration in the culture medium. An uniform polyphosphate labeling was observed on the cellular surface, particularly cell wall and cytoplasmic membrane. Reaction products were visualized in intracellular structures, in the form of trabecules, vacuoles and vesicles, electrondense bodies and disperse granules in the cytoplasm. The results obtained in this study reveal the potential of Cunninghamella elegans for polyphosphate accumulation, and suggest it is possible application in biotechnological processes

ASSUNTO(S)

cunninghamella elegans micologia cunninghamella elegans zygomycetes polyphosphate polifosfato citoquÃmica ultraestrutural zygomycetes ultrastructural cytochemistry

ACESSO AO ARTIGO

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