Animal Cloning
Mostrando 13-24 de 198 artigos, teses e dissertações.
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13. Clonagem, expressão e purificação de proteínas candidatas vacinais de Leptospira interrogans sorovar Copenhageni. Avaliação de atividades imunogênicas e características funcionais. / Cloning, expression and purification of proteins, vaccine candidates from Leptospira interrogans sorovar Copenhageni. Evaluation of immunogenic and functional characteristics.
Leptospirosis is a zoonosis worldwide distributed, caused by bacteria from the genus Leptospira. The development of an efficient vaccine of sub-unities would be of major interest for public health. We proposed to investigate 4 antigens from Leptospira interrogans srv Copenhageni, genes LIC12659, LIC12660, LIC12631 and LIC10868, which were amplified and clone
Publicado em: 2009
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14. The use of cardiotocography as a method of diagnosis of the occurrence of fetal distress (fetal hypoxia) during intrauterine life in fetuses of Nellore generated through the technique of somatic adult cell nuclear transfer - Cloning / O uso da cardiotocografia como método de diagnóstico da ocorrência de sofrimento fetal (hipóxia fetal) durante a vida intrauterina de fetos da raça Nelore originados por meio da técnica de transferência nuclear de células somáticas adultas - Clonagem
This research had the purpose to standardize the technique of cardiotocography to be used to assess the presence of hypoxia / fetal distress during intrauterine life, trying to establish from the 7th month of pregnancy the normal range of fetal heart rate in healthy fetuses and from artificial insemination and cloning to evaluate the influence of the paramet
Publicado em: 2009
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15. Identificação e estudo da diversidade do papilomavírus associado a lesões cutâneas em bovinos
Papillomaviruses (PV) has a worldwide distribution and is recognized as an etiologic agent associated with several benign and malignant epithelia lesions in human and animals. The Papillomaviridae family is a highly heterogeneous group of viruses, which has 18 genera, and likely occurs in most mammals and birds. The PV genome is circular double-stranded DNA
Publicado em: 2008
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16. Diatraea saccharalis larvae midgut β-glicosidases: cDNAs cloning and sequencing, expression and some properties / β-glicosidases intestinais da larva de Diatraea saccharalis: clonagem e sequenciamento dos cDNAs, expressão e algumas propriedades
Utilizando uma bibioteca de expressão feita a partir do epitélio do intestino médio da larva de Diatraea saccharalis, nós clonamos e sequenciamos 3 cDNAs completos (DsβglyA, DsβglyB e DsβglyC), além de uma seqüência parcial que teoricamente codificam β-glicosidases. As sequências dos peptídeos obtidos após digestão por tripsina
Publicado em: 2008
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17. Construction of auxotrophic marker in Mycobacterium bovis BCG, knockout strain for the DPPD and proteomic study of tuberculin / Construção de marcador auxotrófico em Mycobacterium bovis BCG, de uma cepa knockout para DPPD e estudo proteômico da tuberculina
Mycobacterium bovis BCG has the potential to be an effective live vector for multivalent vaccines. However, there are two problems regarding the utilization of recombinant BCG as vaccine. The first one is that most mycobacterial cloning vectors rely on antibiotic resistance gene as selectable marker, which is used for genetic transformation. The second one i
Publicado em: 2008
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18. Caracterização molecular do gene L1 de um provável novo tipo de papilomavírus bovino identificado no Brasil
Papillomaviruses (PVs) comprise a highly diverse group of non-enveloped oncovirus classified in Papillomaviridae family, whose genome consists of a circular double-stranded DNA molecule. Depending on nucleotide identity presented in structural protein L1, 10 types of bovine papillomavirus (BPV) are described in cattle, being each of them located in genera De
Publicado em: 2008
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19. Clonagem de serino proteases do veneno da cascavel Crotalus durissus terrificus e expressão da giroxina em célula de mamífero / Cloning of serine proteases from the venom of rattlesnake Crotalus durissus terrificus and expression of a gyroxin in mammalian cells
As serino proteases participam de diversos processos fisiológicos (tal como o de coagulação) e patológicos. Essas enzimas estão amplamente distribuídas entre as espécies, são também toxinas dos venenos de serpentes, sendo denominadas SVSPs (snake venom serine proteases). Essas SVSPs são multifuncionais e contêm uma tríade catalítica formada pelo
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 05/12/2007
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20. Utilização da actinomicina D como método de enucleação química de ovócitos bovinos destinados à transferência nuclear
Cloning by Nuclear Transfer (NT) is a low efficiency and labor intense technique. The process, in technical terms, is one of the main factors responsible for both embryo quality and quantity. Enucleation is the most aggressive step. Although alternative methods have been proposed, it remains mainly as it has been first described in the early fifties. The obj
Publicado em: 2007
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21. Inducing mutations in the mouse genome with the chemical mutagen ethylnitrosourea
When compared to other model organisms whose genome is sequenced, the number of mutations identified in the mouse appears extremely reduced and this situation seriously hampers our understanding of mammalian gene function(s). Another important consequence of this shortage is that a majority of human genetic diseases still await an animal model. To improve th
Brazilian Journal of Medical and Biological Research. Publicado em: 2006-09
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22. Cloning, expression and characterization of an antigenic domain of the a major surface protein recombinant NcSRS2 (Nc1-p43) of Neospora caninum. / Clonagem, expressão e caracterização do domínio antigênico da proteína majoritária de superfície NcRS2 (Nc1-p43) recombinante de Neospera caninum
Neospora caninum is an obligate intracellular protozoan that can infect domestic and wild canids, as well as ruminants and equines. It was described in 1988 as causing neuromuscular alterations and death in dogs. Recently, N. caninum has received considerable attention for its large impact on the dairy industry, given the economic losses related to breeding
Publicado em: 2006
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23. Patologia e caracterização molecular de Neorickettsia Helminthoeca em cães no Brasil
Neorickettsia helminthoeca, an intracellular obligate bacterium, is the cause of salmon poisoning disease (SPD) of canids. The digenean trematode, Nanophyetus salmincola, is the vector of the bacterium, which maintains the pathogen throughout its entire life cycle, that includes a river snail (Oxytrema silicula), fishes (principally, salmon and trout), and c
Publicado em: 2006
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24. Produção, caracterização e avaliação da imunogenicidade do complexo protéico MSP1 recombinante (rMSP1a erMSP1b) de isolado paranaense do anaplasma marginale
The Anaplasma marginale (Rickettsiales: Anaplasmataceae) is a pathogen that cause bovine anaplasmosis. This disease occurs in world wide, and it is more frequently in tropical and subtropical areas, causing important economic losses in cattle herds. Six Major Surface Protein (MSP1a, MSP1b, MSP2, MSP3, MSP4 and MSP5) have been identified in A. marginale. The
Publicado em: 2006