Identificação e estudo da diversidade do papilomavírus associado a lesões cutâneas em bovinos

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

Papillomaviruses (PV) has a worldwide distribution and is recognized as an etiologic agent associated with several benign and malignant epithelia lesions in human and animals. The Papillomaviridae family is a highly heterogeneous group of viruses, which has 18 genera, and likely occurs in most mammals and birds. The PV genome is circular double-stranded DNA and has 10 ORFs. The PCR assay with degenerate primers that amplifies relatively conserved region in the L1 ORF, followed by sequencing, have been allowed the identification of numerous PV types in human and other animal hosts. The bovine papillomavirus (BPV) are classified in the Deltapapillomavirus (BPV-1 and -2), Epsilonpapillomavirus (BPV-5 and -8), Xipapillomavirus (BPV-3, -4, -6, -9, -10) genera and one unassigned genus (BPV-7). Bovine papillomatosis is extremely frequent throughout the brazilian cattle beef and mainly, dairy herds. However, studies carried out for evaluated either the BPV typing, single or mix infection, and the BPV infection incidence is still infrequent. The aim of the present study is determinate the BPV types and way of infection (single, mix or co-infection) in papilloma specimens in cattle herds from north region of Paraná state. Were analyzed, by PCR assay with degenerate primers pair FAP59/FAP64, twenty-one papilloma specimens from two beef (n=16) and two dairy (n=6) herds. The PCR products were submitted a direct sequencing and the four amplicons which the prior analysis could be determined the BPV type, were cloning and the phylogenetic analysis was performed. PV DNA was detected in all DNA samples from the 22 papilloma specimens evaluated by using FAP PCR, resulting in bands with approximately 480 base pairs. Four BPV types were identified (BPV-1, -2, -6, and -8). The BPV-8, recently described in Asia and Europe, was the first identified in the American continent, suggests, probably, a worldwide distribution. In addition, four putative new BPV types could be identified for the first time. In six animals, which were collected, in distinctly regions of body, more than one papilloma, could be observed mix infection with two (5 animals) or three (one animal) different BPV types. Moreover, we also identified a case of co-infection. In a teat papilloma from adult cow, by cloning and sequencing, were identified in the same lesion, the BPV-1 and BPV-6. Is the first report of these two distinctly forms of BPV infection in Brazil. Our results shown that the papillomavirus infection can be so frequent in bovine as in human beings. In addition, to the epidemiological importance, molecular studies based on the identification of BPV types are necessary to define the appropriate control and prophylaxis measures.

ASSUNTO(S)

papillomaviruses papillomavirus diseases vírus do papiloma bovino - doenças doenças do vírus do papiloma cattle - diseases

ACESSO AO ARTIGO

Documentos Relacionados