Animal Cloning
Mostrando 25-36 de 198 artigos, teses e dissertações.
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25. Produção, purificação, clonagem e aplicação de enzimas líticas
Lytic enzymes such as beta-1,3 glucanases, proteases and chitinases are able to hydrolyse, respectively, beta-1,3 glucans, mannoproteins and chitin, as well as the cell walls of many yeast species. Lytic enzymes are useful in a great variety of applications including the preparation of protoplasts; the extraction of proteins, enzymes, pigments and functional
Química Nova. Publicado em: 2005-10
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26. Bone morphogenetic proteins: from structure to clinical use
Bone morphogenetic proteins (BMPs) are multi-functional growth factors belonging to the transforming growth factor ß superfamily. Family members are expressed during limb development, endochondral ossification, early fracture, and cartilage repair. The activity of BMPs was first identified in the 1960s but the proteins responsible for bone induction were un
Brazilian Journal of Medical and Biological Research. Publicado em: 2005-10
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27. O Efeito de um protocolo de alongamento muscular passivo sobre a cartilagem articular.
Objective: The aim of this study is to evaluate the articular cartilage alterations of rat ankles, after applying unilateral cyclic passive muscle stretching protocol for three weeks in previously immobilized rats. Materials and Methods: Twenty-two male albino rats (280+25.4g) divided into four groups: I-immobilized (n=6), IS-immobilized and stretched (n=5),
Publicado em: 2005
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28. Circovírus suino tipo 2 (PCV2): caracterização molecular e construção de vetores para expressão da proteína do capsídeo / Porcine circovirus type 2 (PCV2): Molecular characterization and vectors construction for capsid protein expression
PCV presents a single-stranded circular DNA genome of approximately 1760 bp, with two main open reading frames (ORFs): ORF1 that encodes viral replicase and ORF2 that encodes viral capsid protein. The aim of this work was to perform a molecular characterization of a PCV2 isolate and produce an expression vector containing the sequence of viral capsid protein
Publicado em: 2005
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29. Embriões, células-tronco e terapias celulares: questões filosóficas e antropológicas
AS THE possibility of therapeutic cloning in humans has just been demonstrated by a Corean group of researchers, the paper argues that biotechnologies of that kind may contribute to alleviate the chronic shortage of organs and tissues for transplantation. Ever since immunosuppressant drugs have become available, transplantation surgery has made considerable
Estudos Avançados. Publicado em: 2004-08
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30. Caracterização de genes de Arabidopsis thaliana e Saccharum spp envolvidos na resposta ao frio e oxido nitrico
Nitric oxide (NO) is a reactive nitrogen species relatively unstable that has great versatility. NO acts as a secondary messenger and may be even cytotoxic, depending on its concentration. Actions of the NO as secondary messenger have been described in neurotransmission, regulation of the blood circulation and inflammatory processes mainly in mammals where t
Publicado em: 2004
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31. "Clonagem em bovinos: uso de fibroblastos fetal e adulto como fonte doadora de núcleo" / Bovine cloning: fetal and adult fibroblasts as nuclei donor source.
O objetivo deste estudo foi avaliar a viabilidade in vitro e in vivo de embriões bovinos reconstruídos com oócitos enucleados em Metáfase II e núcleos de células somáticas (fibroblastos) fetais e adultas. Para tanto, oócitos de ovários colhidos em matadouro foram maturados in vitro por 17 horas e enucleados pela remoção do primeiro corpúsculo pol
Publicado em: 2003
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32. Purificação, caracterização, clonagem e seqüenciamento de β-glicosidades de Tenebrio molitor (Coleoptera) / Purification, characterization, cloning and sequencing of β-glycosidases from Tenebrio molitor (Coleoptera)
In the midgut lumen of Tenebrio molitor larvae there are 4 β-glycosidases (named 1, 2, 3a and 3b), not present in the animal food. They were purified with electrophoresis, ion exchange and hydrophobic chromatographies. β-glycosidase 1 (relative molecular weight - Mr 59,000) is unstable at 30°C but is stabilized by substrates. The enzyme hydrolyses
Publicado em: 2001
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33. Progress toward generating a ferret model of cystic fibrosis by somatic cell nuclear transfer
Mammalian cloning by nuclear transfer from somatic cells has created new opportunities to generate animal models of genetic diseases in species other than mice. Although genetic mouse models play a critical role in basic and applied research for numerous diseases, often mouse models do not adequately reproduce the human disease phenotype. Cystic fibrosis (CF
BioMed Central.
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34. What of animal cloning?
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35. Towards a complete description of the microRNA complement of animal genomes
Recent cloning and computational studies have sought to catalog all the microRNA genes encoded in animal genomes. Here, we highlight recent advances in identifying Caenorhabditis elegans and Drosophila melanogaster microRNAs.
BioMed Central.
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36. Specific genetic modifications of domestic animals by gene targeting and animal cloning
The technology of gene targeting through homologous recombination has been extremely useful for elucidating gene functions in mice. The application of this technology was thought impossible in the large livestock species until the successful creation of the first mammalian clone "Dolly" the sheep. The combination of the technologies for gene targeting of som
BioMed Central.