Heterologous Protein
Mostrando 1-12 de 2182 artigos, teses e dissertações.
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1. Heterologous expression and mutagenesis of recombinant Vespa affinis hyaluronidase protein (rVesA2)
Abstract Background: Crude venom of the banded tiger waspVespa affinis contains a variety of enzymes including hyaluronidases, commonly known as spreading factors. Methods: The cDNA cloning, sequence analysis and structural modelling of V. affinis venom hyaluronidase (VesA2) were herein described. Moreover, heterologous expression and mutagenesis of rVesA2
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 05/12/2019
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2. Antivenomic approach of different Crotalus durissus collilineatus venoms
Abstract Background: Our group has previously performed a proteomic study verifying that individual variations can occur among Crotalus durissus collilineatus venoms. These variations may lead to differences in venom toxicity and may result in lack of neutralization of some components by antivenom. In this way, this study aimed to evaluate the Brazilian ant
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 17/12/2018
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3. Molecular farming of antimicrobial peptides: available platforms and strategies for improving protein biosynthesis using modified virus vectors
Abstract The constant demand for new antibiotic drugs has driven efforts by the scientific community to prospect for peptides with a broad spectrum of action. In this context, antimicrobial peptides (AMPs) have acquired great scientific importance in recent years due to their ability to possess antimicrobial and immunomodulatory activity. In the last two dec
An. Acad. Bras. Ciênc.. Publicado em: 18/10/2018
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4. Parenteral adjuvant potential of recombinant B subunit of Escherichia coli heat-labile enterotoxin
BACKGROUND The B subunit of Escherichia coli heat-labile enterotoxin (LTB) is a potent mucosal immune adjuvant. However, there is little information about LTB's potential as a parenteral adjuvant. OBJECTIVES We aimed at evaluating and better understanding rLTB's potential as a parenteral adjuvant using the fused R1 repeat of Mycoplasma hyopneumoniae P97 ad
Mem. Inst. Oswaldo Cruz. Publicado em: 2017-12
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5. Heterologous expression, protein folding and antibody recognition of a neurotoxin from the Mexican coral snake Micrurus laticorallis
Abstract Background The cysteine-rich neurotoxins from elapid venoms are primarily responsible for human and animal envenomation; however, their low concentration in the venom may hamper the production of efficient elapid antivenoms. Therefore, the aim of the present study was to produce fully active elapid neurotoxic immunogens for elapid antivenom produc
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 13/10/2016
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6. A comparison between the recombinant expression and chemical synthesis of a short cysteine-rich insecticidal spider peptide
Abstract Background: The choice between heterologous expression versus chemical synthesis for synthesizing short cysteine-rich insecticidal peptides from arthropods may impact the obtainment of yields and well-folded bioactive molecules for scientific research. Therefore, two recombinant expression systems were compared to
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 11/08/2015
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7. Alzheimer's disease: is a vaccine possible?
The cause of Alzheimer's disease is still unknown, but the disease is distinctively characterized by the accumulation of β-amyloid plaques and neurofibrillary tangles in the brain. These features have become the primary focus of much of the research looking for new treatments for the disease, including immunotherapy and vaccines targeting β-amyloid in the
Braz J Med Biol Res. Publicado em: 2014-06
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8. HSF-1, HIF-1and HSP90 expression on recombinant Pichia pastoris under fed-batch fermentation
Pichia pastoris is a methylotrophic yeast used as an efficient expression system for heterologous protein production as compared to other expression systems. Considering that every cell must respond to environmental changes to survive and differentiate, determination of endogenous protein related to heat stress responses and hypoxia, it would necessary to es
Braz. J. Microbiol.. Publicado em: 2014-06
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9. Biomarkers to evaluate the effects of temperature and methanol on recombinant Pichia pastoris
Pichia pastoris is methylotrophic yeast used as an efficient expression system for heterologous protein production. In order to evaluate the effects of temperature (10 and 30 °C) and methanol (1 and 3% (v/v)) on genetically-modified Pichia pastoris, different biomarkers were evaluated: Heat stress (HSF-1 and Hsp70), oxidative stress (OGG1 and TBARS) and ant
Braz. J. Microbiol.. Publicado em: 2014-06
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10. Determinação da atividade antitríptica em proteínas de produtos do amendoim isoladas por cromatografia de afinidade
The peanut is an oleaginous plant of high nutritional value, a source of protein and a trypsin inhibitor. Trypsin inhibitors are proteins present in the vegetable kingdom, considered anti-nutritional factors for animals. However, there have been several recent reports about their heterologous and beneficial effects on human health. These important effects ha
Quím. Nova. Publicado em: 2014
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11. Construção de cassetes de expressão lineares e transformação de Kluyveromyces marxianus para expressão de proteínas heterólogas / Construction of linear expression cassetes na transformation of Kluyveromyces marxianus for heterologous protein expression
A escolha da levedura hospedeira para produção de uma proteína heteróloga é fundamental para o sucesso de um processo biotecnológico, porém, apesar da diversidade de trabalhos com expressão de proteínas em leveduras, os níveis de secreção nesses organismos ainda são considerados baixos quando comparadas aos fungos filamentosos. A levedura K. mar
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 15/02/2012
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12. Experimental model of heterotopic ossification in Wistar rats
Heterotopic ossification (HO) is a metaplastic biological process in which there is newly formed bone in soft tissues adjacent to large joints, resulting in joint mobility deficit. In order to determine which treatment techniques are more appropriate for such condition, experimental models of induced heterotopic bone formation have been proposed using hetero
Brazilian Journal of Medical and Biological Research. Publicado em: 2012-06