Heterologous Protein
Mostrando 13-24 de 2182 artigos, teses e dissertações.
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13. Soybean genetic transformation: a valuable tool for the functional study of genes and the production of agronomically improved plants
Transgenic plants represent an invaluable tool for molecular, genetic, biochemical and physiological studies by gene overexpression or silencing, transposon-based mutagenesis, protein sub-cellular localization and/or promoter characterization as well as a breakthrough for breeding programs, allowing the production of novel and genetically diverse genotypes.
Genet. Mol. Biol.. Publicado em: 2012
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14. Clonagem, expressão heteróloga e caracterização da proteína de escolta da Hsp70 de Leishmania braziliensis / Cloning, heterologous expression and characterization of protein Hsp70 escort of Leishmania braziliensis
A Leishmaniose é uma doença infecciosa causada por protozoários flagelados do gênero Leishmania. Os parasitas como a Leishmania braziliensis, sofrem várias mudanças morfológicas durante seu ciclo de vida, incluindo a troca de organismo hospedeiro. Durante essas mudanças, proteínas de choque térmico ou chaperones moleculares, como, por exemplo, a Hs
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 17/08/2011
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15. Expressão heteróloga do domínio III da proteína de envolope (E) do vírus dengue-1 e 2 em Pichia pastoris / Heterologous expression of domain III of the envolope (E) protein of dengue virus-1 and 2 in Pichia pastoris
Anualmente os vírus dengue (DENV) são responsáveis por 100 milhões de infecções em todo o mundo. O vírus apresenta quatro sorotipos diferentes, DENV-1, 2, 3 e 4, e é constituído por três proteínas estruturais (C, prM e E) e sete nãoestruturais. A proteína de envelope (E) é responsável pela ligação do vírus à célula hospedeira, sendo compo
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 08/06/2011
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16. ExpressÃo de uma quitinase de Chromobacterium Violaceum em Pichia Pastoris: purificaÃÃo e caracterizaÃÃo parcial da proteÃna recombinante / Expression of a chitinase from Chromobacterium Violaceum in Pichia pastoris: purification and partial characterization of recombinant protein
A utilizaÃÃo de microrganismos como sistemas heterÃlogos de expressÃo de proteÃnas tem se mostrado uma estratÃgia alternativa e/ou complementar aos passos tradicionais utilizados no processo de purificaÃÃo de proteÃnas. Diante deste contexto, quitinases (EC 3.2.1.14), enzimas hidrolÃticas capazes de degradar quitina, tÃm sido expressas em diferent
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 28/02/2011
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17. Production of L1 protein from different types of HPV in Pichia pastoris using an integrative vector
Human papillomavirus (HPV) infection is the most common sexually transmitted disease in the world and is related to the etiology of cervical cancer. The most common high-risk HPV types are 16 and 18; however, the second most prevalent type in the Midwestern region of Brazil is HPV-33. New vaccine strategies against HPV have shown that virus-like particles (V
Brazilian Journal of Medical and Biological Research. Publicado em: 2011-12
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18. Synergism/complementarity of recombinant adenoviral vectors and other vaccination platforms during induction of protective immunity against malaria
The lack of immunogenicity of most malaria antigens and the complex immune responses required for achieving protective immunity against this infectious disease have traditionally hampered the development of an efficient human malaria vaccine. The current boom in development of recombinant viral vectors and their use in prime-boost protocols that result in en
Memórias do Instituto Oswaldo Cruz. Publicado em: 2011-08
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19. Clonagem e expressão do gene da tiorredoxina 1 de Paracoccidioides brasiliensis em Pichia pastoris / Cloning and expression of the thioredoxin 1 gene of Paracoccidioides brasiliensis in pichia pastoris
The termodimorphic fungus Paracoccidioides brasiliensis is the etiological agent of paracoccidioidomycosis, a human systemic mycosis of high prevalence in Latin America. P. brasiliensis is exposed to oxidative stress (OS) caused by reactive oxygen species (ROS) produced by the defense cells of the human host. When the invasion by pathogens occurs, the host d
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 27/08/2010
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20. Efeito de candidatos a supressores de silenciamento gênico viral em expressão de proteína recombinate em plantas
The aim of this work was to increase the efficiency of transient heterolog protein expression system using viral gene-silencing suppressors and Agrobacterium integrated. The use of plants as bioreactor is a promising strategy for large scale production of the heterolog protein. However, the use of genetically modified plants may lead to low protein expressio
Publicado em: 2010
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21. Título Principal Toxinas recombinantes Cry2Aa e Cry11A de Bacillus thuringiensis expressas em células de inseto são tóxicas para larvas de Lepidoptera e Diptera
Bacillus thuringiensis (Bt) is a Gram positive, tomopathogenic bacteria that produces crystalline inclusions called !-endotoxins or Cry proteins. These proteins are highly toxic to susceptible insects, not showing activity against others organisms. Some expression systems have been used to express crystal proteins with the aim to increase their toxicity for
Publicado em: 2009
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22. Expression of schistosomal cathepsin l1 in Escherichia coli and evaluation of its protective capacity in an animal challenge
Schistosomes utilize proteinases to accomplish several activities such as tissue penetration, tissue digestion and evasion of host immune responses. Cathepsin L is a cysteine proteinase of the papain superfamily detected in their gut lumen which indicates that this enzyme contributes to the proteolysis of ingested hemoglobin. Due to the roles played in the s
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2009
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23. Use of Kluyveromyces lactis in the Expression of Leishmania major s GDPase. / Utilização de Kluyveromyces lactis na expressão da GDPase de Leishmania major.
The yeast Kluyveromyces lactis has a potential applicability as a host in biotechnological production of heterologous recombinant proteins of biotechnological interest. In this context the objective of this work was to express the protein of Leishmania major GDPase for biotechnology applied to human and canine leishmaniasis. The GDPase of L. major belongs to
Publicado em: 2009
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24. Clonagem, expressão heteróloga e caracterização molecular de um gene de lacase de Pycnoporus sanguineus
Lacases are enzymes with potential application in a set of industrial processes. Some authors described their use in bioremediation of phenolic compounds of pharmaceutical and dyes industries and in the removal of lignin of the cellulose pulp. In this study a laccase from Pycnoporus sanguineus previously described by Garcia and co-workers 2006), was expresse
Publicado em: 2009