Gel Eletrophoresis
Mostrando 13-24 de 25 artigos, teses e dissertações.
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13. Análise proteômica comparativa entre neutrófilos não ativados e ativados com PMA, um análogo do diacilglicerol
Neutrophils are the major type of leukocytes in peripheral blood and protect against fungal and bacterial infections. Directed migration in a gradient of chemotactic stimuli enables these cells to rapidly find the site of infection and destroy the invading pathogens. PMNs can exist in three different activation states, namely, resting, primed, and activated
Publicado em: 2007
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14. Identification of differently expressed genes associated with Citrus Blight (Citrus Spp.). / IdentificaÃÃo de genes diferencialmente expressos associados ao declÃnio dos citros (Citrus spp).
O Brasil à o maior produtor de citros do mundo, sendo responsÃvel por mais de 20% da produÃÃo mundial. No entanto, a produÃÃo ainda à baixa devido a muitos problemas fitossanitÃrios, a exemplo do DeclÃnio dos Citros. O DeclÃnio dos Citros à uma anomalia cuja causa ainda nÃo foi determinada. As Ãrvores que comeÃam a declinar nÃo se recuperam, c
Publicado em: 2007
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15. Proteinograma de caprinos da raça Pardo-Alpina infectados naturalmente por parasitos gastrintestinais
The proteinogram of six 12 month-old Alpine goats, intensively raised and naturally infected by gastrointestinal parasites, was evaluated. Blood and feces samples of each animal were monthly collected. Total serum protein and their fractions were determined by agarose gel eletrophoresis, using Tris buffer, pH 9.2. The identified protein fractions were albumi
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Publicado em: 2006-04
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16. Genotypic characterization of extended-spectrum beta-lactamase-producing Enterobacteriaceae strains, isolated from patients of a public hospital in the city of São Paulo. / Caracterização genotípica de cepas da família enterobacteriaceae produtoras de ß-lactamases de espectro estendido, isoladas de pacientes de um hospital da rede pública da cidade de São Paulo.
Introdução - A crescente resistência antimicrobiana em bactérias responsáveis por infecções hospitalares é um grande desafio à Saúde Pública. as B-lactamases de espectro estendido (ESBL), que hidrolisam a maioria dos compostos B-lactâmicos, são reconhecidas mundialmente como um grande problema para pacientes hospitalizados, devido à localizaç�
Publicado em: 2006
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17. Prevalence of deltaF508, G551D, G542X, and R553X mutations among cystic fibrosis patients in the North of Brazil
Cystic fibrosis (CF) is the most common genetic disease among Caucasians and is rare among sub-Saharan Africans. The Brazilian population is not ethnically homogeneous but it is the result of three-way ethnic admixture of Europeans, Africans and Amerindians in varying proportions, depending on the region. In the present study, we investigated 33 patients who
Brazilian Journal of Medical and Biological Research. Publicado em: 2005-01
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18. Characterization of extracellular proteases produced by Xylella fastidiosa from citrus and grapevines. / Caracterização de proteases extracelulares produzidas por Xylella fastidiosa de citros e videira.
Xylella fastidiosa is a pathogenic bacterium found in several plants. These bacteria secrete extracellular proteases into the culture broth as visualized in sodium-dodecyl-sulfate polyacrylamide activity gels containing gelatin as a co-polymerized substrate. Three major protein bands were produced by strain X0 (citrus) with molar masses (MM) of 122, 84 and 6
Publicado em: 2005
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19. Polymorphisms in MSX1 gene can be related to tood agenesis in a Brazilian population / Analise de polimorfismos na região de intro do gene MSX1 em individuos com hipodontia
A ausência congênita de um ou mais dentes, denominada agenesia dentária ou hipodontia, figura entre as anomalias mais bem reconhecidas em humanos, porém de etiologia ainda desconhecida. Os dentes permanentes mais comumente ausentes são: terceiros molares, segundos pré-molares e incisivos laterais superiores. A hipodontia não representa um sério probl
Publicado em: 2005
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20. Molecular approaches to detect cyanobacteria and their microcystins producing genotypes in Billings and Guarapiranga reservoirs, São Paulo, Brazil / Abordagens moleculares para detectar cianobactérias e seus genótipos produtores de microcistinas presentes nas represas Billings e Guarapiranga, São Paulo, Brasil
Cyanobacterial blooms in water reservoirs used for human consumption are frequent nowadays and are usually attributed to the increasing water eutrophization. In recent years, the appearance of toxin-producing cyanobacterial strains has concerned managers of water quality since these toxins represent a public health risk. Early detection of the presence of to
Publicado em: 2004
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21. Polimorfismo dos genes PSGL-1, ICAM1, CD18, mieloperoxidade e manifestações clinicas na anemia falciforme
Anemia falciforme (AF) é uma doença monogênica, mas com uma apresentação clínica variável, devido a possível associação com outros genes. Especula-se que nesta doença genes envolvidos com a resposta inflamatória tenham efeito epistático. A mieloperoxidase (MPO) é uma enzima com atividade antimicrobicida, sendo encontrada nos grânulos azurofili
Publicado em: 2004
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22. Phylogenetic relationships among four species of the guarani group of Drosophila (Diptera, Drosophilidae) as inferred by molecular and morphological analyses
Traditionally, the Drosophila guarani species group has been divided into two subgroups: the guarani and the guaramunu subgroups. Two, out of the four species included in this research, are members of the guarani subgroup (D. ornatifrons Duda, 1927 and D. subbadia Paterson & Mainland, 1943) and two are included in the guaramunu subgroup (D. maculifrons Duda,
Revista Brasileira de Entomologia. Publicado em: 2002
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23. Activity analiysis of some antioxidant enzymes in soybean plants (Glycine max L. Merr.) under levels of manganese, in function of arbuscular mycorrhizae. / Análise da atividade de algumas enzimas antioxidantes em plantas de soja (Glycine max L. Merr.) sob níveis de manganês, em função da micorriza arbuscular.
Brazilian soils are predominantly acid, which is considered a common incident in tropical regions. Soil acidity in combination with biotic and non-biotic factors can result manganese toxicity in plants, limiting their development. The use of conventional agricultural practices promotes an increase in Mn toxicity, as it reduces the soil content of organic mat
Publicado em: 2002
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24. Expression and Characterization of Recombinant Human-Derived Pneumocystis carinii Dihydrofolate Reductase
Dihydrofolate reductase (DHFR) is the target of trimethoprim (TMP), which has been widely used in combination with sulfa drugs for treatment and prophylaxis of Pneumocystis carinii pneumonia. While the rat-derived P. carinii DHFR has been well characterized, kinetic studies of human-derived P. carinii DHFR, which differs from rat-derived P. carinii DHFR by 3
American Society for Microbiology.