Characterization of extracellular proteases produced by Xylella fastidiosa from citrus and grapevines. / Caracterização de proteases extracelulares produzidas por Xylella fastidiosa de citros e videira.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

Xylella fastidiosa is a pathogenic bacterium found in several plants. These bacteria secrete extracellular proteases into the culture broth as visualized in sodium-dodecyl-sulfate polyacrylamide activity gels containing gelatin as a co-polymerized substrate. Three major protein bands were produced by strain X0 (citrus) with molar masses (MM) of 122, 84 and 65 kDa. Grape strain 9713 produced two bands of approximately 84 and 64 kDa. These organisms produced zones of hydrolysis in agar plates amended with gelatin, casein and hemoglobin. Gelatin was the best substrate for these proteases. SDS-PAGE activity gel indicated that the protease activities of X. fastidiosa from citrus and grape were completely inhibited by PMSF and partially inhibited by EDTA. The optimal temperature for protease activity was 30oC with an optimal pH of 7.0. Among the proteolytic enzymes secreted by the phytopathogen, chitinase and β-1,3-glucanase activities were also detected in cultures of X. fastidiosa (citrus). From these results, it is suggested that these proteases produced by strains of X. fastidiosa from citrus and grape, belong to the serine- and metallo-protease group.

ASSUNTO(S)

enzyme extracellular uva eletroforese eletrophoresis enzima proteolítica bactéria patogênica enzima extracelular grape protein fruta cítrica proteolytic enzymes phytopathogenic bacteria citric fruit proteína enzyme inhibitor inibidor de enzima

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