Urinary porphyrins reference ranges by HPLC in a population not exposed to porphyrinogenic agents / Valores de referência de porfirinas urinárias por CLAE numa população não-exposta a agentes químicos porfirinogênicos

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

Porphyrins which are formed as intermediates in heme biosynthesis, vary from eight to four carboxyl groups: uro, hepta, hexa, penta and coproporphyrins. Alterations in the urinary porphyrin excretion profile may be caused by a hereditary disease or by environmental/occupational exposure. The purpose of this study was to establish a sensitive and accurate highperformance liquid chromatography (HPLC) method with fluorescence detection to estimate reference ranges of urinary porphyrin fractions in a population of São Paulo city, Brazil. Random urine samples were collected from 126 subjects (18 - to 65-year-old) of both sexes not occupationally exposed to porphyrinogenic agents such as chlorinated hydrocarbons, As, Hg and Pb. Distribution were nonparametric and reference ranges obtained in µg/g of creatinine, mean ± SD and 2,5 -97,5 th percentile were: for 8- carboxyl (uro): 5,3 ± 6,0 and 0 - 20,8; for 4-carboxyl (copro): 42,8 ± 26,4 and 7,4-133,6 and for total porphyrins: 48,1 ± 27,7 and 7,4 -159,4 respectively. Hepta, hexa and pentaporphyrins were not quantified. No statistically significant correlation was found for sex and age. Smokers had lower levels of copro and total porphyrins statistically significant (median 37,19 and 43,48) than nonsmokers (median 22,49 and 27,18). The proposed method, which allows for the detection of minor alterations in porphyrin excretion and the reference ranges estimated are potentially applicable as biological markers in exposure to porphyrinogenic agents.

ASSUNTO(S)

reference range agentes porfirinogênicos intervalo de referência toxicologia ocupacional urinary porphyrins porphyrinogenic agents porfirinas urinárias

ACESSO AO ARTIGO

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