Transferência gênica in vivo por meio de eletroporação em músculo sóleo de ratos wistar. / In vivo gene transfer by electroporation in soleus muscle from wistar rats.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

The use of electrical pulses for gene transfer has been successfully used to reach significant levels of gene expression in vitro and in vivo. Therefore electroporation can be considered an important device to get further insight on biological mechanisms and also to treatment. The aim of the present work is to achieve a high level of gene transfer throughout electroporation in the soleus muscle of Wistar rats. The muscle was surgically accessed, injected with Hyaluronidase and subsequently injected with a plasmid expressing GFP (Green Fluorescence Protein). Observation of fluorescence produced in situ and also HE (Hematoxilin-eosin) preparations revealed that the best gene transfer efficiency (50, 3 ± 20%) was achieved with 25 V and 8 pulses (1Hz) lasting 20ms. These results point to a potential use of electroporation as a tool for investigating cellular and molecular aspects of skeletal muscle plasticity, especially those that can be approached only in soleus muscle, such as longitudinal growth.

ASSUNTO(S)

gene transfer skeletal muscle plasmídeos proteínas de fluorescência verde soleus green fluorescence protein eletroporação eletroporation músculo esquelético plasmid sóleo transferência de genes

ACESSO AO ARTIGO

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