Gene Transfer
Mostrando 1-12 de 4128 artigos, teses e dissertações.
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1. New tools for cell reprogramming and conversion: Possible applications to livestock
Abstract Somatic cell nuclear transfer and iPS are both forms of radical cell reprogramming able to transform a fully differentiated cell type into a totipotent or pluripotent cell. Both processes, however, are hampered by low efficiency and, in the case of iPS, the application to livestock species is uncertain. Epigenetic manipulation has recently emerged a
Anim. Reprod.. Publicado em: 28/11/2019
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2. Reproductive physiology of the heat-stressed dairy cow: implications for fertility and assisted reproduction
Abstract Heat stress causes a large decline in pregnancy success per insemination during warm times of the year. Improvements in fertility are possible by exploiting knowledge about how heat stress affects the reproductive process. The oocyte can be damaged by heat stress at the earliest stages of folliculogenesis and remains sensitive to heat stress in the
Anim. Reprod.. Publicado em: 28/11/2019
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3. Use of two new formulations as bovine embryo manipulation solution
Abstract This study aimed to evaluate the effect of two Embryo Manipulation Solutions (EMS and EMS supplemented) in maintenance of the viability of embryos, initially using structures derived from mice (first phase). Next, the efficiency of these solutions in routines of bovine embryo transfer was evaluated (second stage). Mice embryos were used in the stage
Anim. Reprod.. Publicado em: 25/11/2019
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4. Earliest identification of New Delhi metallo-β-lactamase 1 (NDM-1) in Acinetobacter pittii in Brazil
Abstract We report the occurrence in Brazil of the bla NDM-1 gene in Acinetobacter pittii, prior to the previously described first reports regarding the species Providencia rettgeri and Enterobacter hormaechei. Clinical isolates were investigated by polymerase chain reaction followed by bidirectional sequencing, and species was confirmed by 16S rDNA sequenci
Rev. Soc. Bras. Med. Trop.. Publicado em: 27/06/2019
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5. Analysis of tRNA halves (tsRNAs) in serum from cattle challenged with bovine viral diarrhea virus
Abstract Acute infections of bovine viral diarrhea virus (BVDV) lead to a range of clinical presentations. Laboratory tests for detection depend on collection of samples during a short viremia. Acutely infected animals remain largely undiagnosed. Transfer RNA halves (tsRNAs) are hypothesized to function like microRNAs to regulate gene expression during an im
Genet. Mol. Biol.. Publicado em: 27/06/2019
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6. Protective effect of Schisandra chinensis total lignans on acute alcoholic-induced liver injury related to inhibiting CYP2E1 activation and activating the Nrf2/ARE signaling pathway
ABSTRACT Schisandra chinensis (Turcz.) Baill., Schisandraceae, is a well-known traditional Chinese medicine used mainly as a recipe for hepatoprotection. Modern researches have revealed that the hepatoprotection is related to its lignans and crude polysaccharide. In this study, we examined the effect and mechanism of S. chinensis total lignans on the liver i
Rev. bras. farmacogn.. Publicado em: 27/05/2019
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7. Exploring tRNA gene cluster in archaea
BACKGROUND Shared traits between prokaryotes and eukaryotes are helpful in the understanding of the tree of life evolution. In bacteria and eukaryotes, it has been shown a particular organisation of tRNA genes as clusters, but this trait has not been explored in the archaea domain. OBJECTIVE Explore the occurrence of tRNA gene clusters in archaea. METHO
Mem. Inst. Oswaldo Cruz. Publicado em: 07/01/2019
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8. Perspectives for cancer immunotherapy mediated by p19Arf plus interferon-beta gene transfer
While cancer immunotherapy has gained much deserved attention in recent years, many areas regarding the optimization of such modalities remain unexplored, including the development of novel approaches and the strategic combination of therapies that target multiple aspects of the cancer-immunity cycle. Our own work involves the use of gene transfer technology
Clinics. Publicado em: 06/09/2018
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9. pMEX01, a 70 kb plasmid isolated from Escherichia coli that confers resistance to multiple β-lactam antibiotics
Abstract Multidrug-resistant microorganisms are of great concern to public health. Genetic mobile elements, such as plasmids, are among the most relevant mechanisms by which bacteria achieve this resistance. We obtained an Escherichia coli strain CM6, isolated from cattle presenting severe diarrheic symptoms in the State of Querétaro, Mexico. It was found t
Braz. J. Microbiol.. Publicado em: 2018-09
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10. pGVG: a new Gateway-compatible vector for transformation of sugarcane and other monocot crops
Abstract The successful development of genetically engineered monocots using Agrobacterium-mediated transformation has created an increasing demand for compatible vectors. We have developed a new expression vector, pGVG, for efficient transformation and expression of different constructs for gene overexpression and silencing in sugarcane. The pCAMBIA2300 bin
Genet. Mol. Biol.. Publicado em: 11/06/2018
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11. Genomic analysis of bifunctional Class C-Class D β-lactamases in environmental bacteria
β-lactamases, which are found in several bacterial species and environments, are the main cause of resistance to β-lactams in Gram-negative bacteria. In 2009, a protein (LRA-13) with two β-lactamase domains (one class C domain and one class D domain) was experimentally characterised, and an extended action spectrum against β-lactams consistent with two f
Mem. Inst. Oswaldo Cruz. Publicado em: 28/05/2018
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12. Genome-wide analysis of the Glycerol-3-Phosphate Acyltransferase (GPAT) gene family reveals the evolution and diversification of plant GPATs
Abstract sn-Glycerol-3-phosphate 1-O-acyltransferase (GPAT) is an important enzyme that catalyzes the transfer of an acyl group from acyl-CoA or acyl-ACP to the sn-1 or sn-2 position of sn-glycerol-3-phosphate (G3P) to generate lysophosphatidic acids (LPAs). The functional studies of GPAT in plants demonstrated its importance in controlling storage and membr
Genet. Mol. Biol.. Publicado em: 19/03/2018