Supplementation effects of whey proteins in lymphocytes of Wistar rats. / O efeito da suplementação de soro de leite sobre os linfocitos de ratos Wistar.

AUTOR(ES)

Laura Andrade Cavalheiro

DATA DE PUBLICAÇÃO

2007

RESUMO

The whey proteins have demonstrated some beneficial effects, as anticarcinogenic activity, antiulcerogenic effect and immunostimulanting function. These immunostimulanting properties have being related to the possible manipulation of intracellular glutathione. The lymphocytes are important cells of the cellular and humoral defense system. The aim of this work was to study the effect of the whey proteins supplementation on lymphocytes of Wistar rats. One hundred and twenty animals were randomly distributed in 4 groups (n = 30): one control group (C) while the others were supplemented with calcium caseinate (K), whey protein isolated (I) and whey protein hydrolysate (H). The rats were maintained in individual cages with water and ration ad libitum. After 18 days of supplementation, blood samples were collected for hemogram accomplishment. Ten days later, the animals were killed and the mesenteric lymphocytes were counted in Neubauer?s camara and incubated (5x107células/ml) in the presence of hydrogen peroxide. The cellular viability was evaluated before and after the incubation. The enzymatic activities of glutathione reductase (GSH-Red) and of glutathione peroxidase (GSH-Px) were also evaluated. Animals of group C had the highest feed consumption, although its body weight gain was the same as group H, greater than group K and lesser than group I. The group H animals have presented number of lymphocytes 134% greater when compared with groups I and K and 158% more than group C animals. The blood analysis have shown that the number of lymphocytes (x103 cells/mL) of group H animals were bigger than the others groups.

ASSUNTO(S)

antioxidantes antioxidants hydrogen peroxide soro do leite supplementation hidrolisados de proteina whey protein hydrolysate glutathione agua oxigenada glutationa

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