Eletroporation
Mostrando 1-5 de 5 artigos, teses e dissertações.
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1. Estudo dos efeitos endócrinos e parácrinos do hormônio de crescimento após administração de DNA plasmidial em modelos animais de terapia gênica / Study of endocrine and paracrine effects of growth hormone after administration of plasmid DNA in animal models of gene therapy
Nosso grupo tem estudado uma estratégia alternativa de tratamento para a deficiência de hormônio de crescimento (DGH), utilizando a administração in vivo do plasmídeo pUC-UBI-hGH, seguida de eletroporação, no músculo quadríceps de camundongos anões e imunodeficientes (lit/scid), que proporcionou níveis sustentáveis de hGH na circulação durante
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 10/06/2011
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2. Transformação de plantas.
ABSTRACT: During centuries mankind plants to obtain cultivars with superior characteristics. However, conventional methods of plant breeding present limitations. The development of transformation methods created possibilities of great progresses in plant breeding because it offers the opportunity to introduce exogenous genes into superior cultivars to obtain
Planaltina. Publicado em: 2011
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3. Development and evaluation of residual virulence of a mutant strain of Brucella Abortus / Desenvolvimento e avaliação da virulência residual de uma cepa mutante de Brucella abortus
Brucella spp. is an intracellular facultative gram-negative bacteria which is pathogenic for many species of mammals, causing brucelosis, a worldwide spread zoonosis. Therefore the search for more efficient alternatives of control, as the development of new strains that can be tested as potential immunogens, is necessary. In this study, we knockouted virB10
Publicado em: 2009
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4. Transferência gênica in vivo por meio de eletroporação em músculo sóleo de ratos wistar. / In vivo gene transfer by electroporation in soleus muscle from wistar rats.
The use of electrical pulses for gene transfer has been successfully used to reach significant levels of gene expression in vitro and in vivo. Therefore electroporation can be considered an important device to get further insight on biological mechanisms and also to treatment. The aim of the present work is to achieve a high level of gene transfer throughout
Publicado em: 2008
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5. Isolamento de análogos de genes de resistência (RGAs) em arachis cardernasii
Wild species of Arachis have been characterized by their high level of genetic polymorphism. Included among these species is Arachis cardenasii that is well known for showing resistances to different diseases. The gene products that are responsable for the resistance to various species of plants have conserved domains, and these domains can be used as molecu
Publicado em: 2002