PurificaÃÃo parcial de uma lectina da raiz de Guettarda platypoda

AUTOR(ES)
DATA DE PUBLICAÇÃO

2003

RESUMO

Lectins, proteins or glycoproteins that reversible bind carbohydrates with distinct degrees of selectivity, they are found in a variety of organisms being involved in numerous cellular processes. In this work evaluated the presence of lectin(s) from Guettarda platypoda D.C., plant popular know with âangelicaâ. Roots of this plant have been used in folk medicine for treatment of different infirmities. Crude extracts (10%, w/u) in 0,15 M NaCl of roots as a whole, root bast, leaves, flowers, fruits, peduncles, petioles, stems, branches of stems and buds were obtained; root bast extracts were even so evaluated in different buffers and pH values (citrate-phosphate pH 3.0-7.0; TRIS-HCl pH 7.5-9.0; sodium-phosphate pH 6.0-7.5). The extracts were submitted to ammonium sulphate fractionation (F 0-60%), dialyse and hemaglutinating assays (HA) with different types of erythrocytes. All evaluated tissues showed activity hemaglutinating; leaves and buds revealed the highest HA specific (SHA) with glutaraldehyde rabbit erytrocytes. The selected fraction with root bast lectin (GplaRL) was obtained in 0.001 M citrate-phosphate buffer (pH 5.5) containing 0.15 M sodium chloride. HA was partial inhibited by glucose and sucrose. The purity degree was evaluated through polyacrylamide gel electrophoresis (PAGE) to acidic and native proteins or denatured/reduced polypeptides; two bands were revealed. GplaRL was adsorved in chromatographic column chitin; one peak was obtained, which reveled one band in PAGE to acidic and native protein. GplaRL seems to be the first lectin mentioned in the genus Guettarda

ASSUNTO(S)

afinidade cromatogrÃfica root lectin affinity chromatography guettarda platypoda bioquimica guettarda platypoda lectina de raiz

ACESSO AO ARTIGO

Documentos Relacionados