PurificaÃÃo da lectina de sementes de Moringa oleifera(WSMoL) e avaliaÃÃo da genotoxidade

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

The work reports the isolation and characterization of Moringa oleifera seed lectin (WSMoL). Its effects on water turbidity as well as genotoxic/mutagenic potential of water treated with different concentrations of M. oleifera seeds (MoW1, MoW2, MoW3 and MoW4) or WSMoL were also discussed. WSMoL, agglutinated different erythrocytes, was mainly active at pH 6.5 to 7.5 and inhibited by azocasein. Gel filtration chromatography and electrophoretic profiles revealed WSMoL as 102 kDa protein constituted by 5 kDa subunits. Glycosylation was not detected. WSMoL had coagulant property similar to the most commonly used coagulant alluminum sulfate. MoW preparations and WSMoL were negative in a cell-free plasmid DNA test, bacterial transformation assay and in Salmonella typhimurium assay using TA97, TA98 and TA100 at presence or absence of hepatic metabolization. MoW1 and MoW2 were also negative by Allium cepa assay however micronucleus formation was induced by MoW3 and MoW4. The results to suggest WSMoL is an active component in the water purification process by seeds. WSMoL was not a genotoxic agent but genotoxicity was detected in water treated with more concentration of seeds. This study is a positive step forward in determining the safe use of M. oleifera seeds for water treatment

ASSUNTO(S)

moringa oleifera mutagenicity genotoxicidade genotoxicity mutagenicidade moringa oleifera bioquimica

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