Estudo bioquimico e ultraestrutural da matriz extracelular durante atrofia experimental de glandulas submandibulares de ratos

AUTOR(ES)
DATA DE PUBLICAÇÃO

1996

RESUMO

Obstructive atrophy of salivary glands is a common disease. It may occur by ductal obstruction caused by calculus, infection or neoplastic processes. In the present work we have used electron microscopy and biochemical methods to study the changes that occur in the expression laminin, type IV collagen and metaloproteinases during the experimental atrophy of the submandibular gland in rats. Immunohistochemical studies of laminin and type IV collagen showed an irregular distribution of there proteins around atrophies ducts and acini. The composition of laminin chains was not altered during glandular atrophy. Western blot analysis showed that rat submandibular gland laminin contain B chains, while A chain seems to be present only in small amounts. Investigation of the expression of fibroblast collagenase (MMP1), by zimographic analysis showed. high levels of this enzyme after 5, days of ligation. Gelatinolytic activity decrease progressively until 30 days of atrophy. Electron microscopy showed extensive morphologic changes in glandular cells. Myoepithelial cells showed "wrinkling" of outer plasma membrane leading to a thickening of the associated basement membrane. We conclude that the atrophy of salivary gland is an useful in vivo model to study the molecular events that take part in the remodeling of the extracellular matrix

ASSUNTO(S)

matriz extracelular salivary glands glandulas salivares metaloproteinas matrix extracellular metalloproteins

ACESSO AO ARTIGO

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