DistribuiÃÃo e anÃlise quantitativa de neurÃnios imunoreativos à enzima triptofano hidroxilase em nÃcleos de rafe de ratos desnutridos durante a vida perinatal / Distribution and quantitative analysis of neurons immunoreactive for the enzyme tryptophan hydroxylase in the raphe nuclei of animals malnourished during perinatal life

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

Nutritional disorders occurring during the development of the nervous system may program the fetal metabolism so long to allow the survival of individuals to changes in the nutritional environment. The perinatal protein malnutrition promotes neurochemical changes, including the levels of serotonin and limiting enzyme for its synthesis, tryptophan hydroxylase (TPH). Virgin rats of the Sprague-Dawley strain received normal diet (control group, C) or hypoproteic (malnourished group, D) during pregnancy and lactation. The pups were fed with normal diet after weaning and subjected to experimentation at 35 days of life. Normonutridos and malnourished pups were acute injection of fenfluramine (3mg/Kg pc) or saline (NaCl 0.9%) forming the saline control groups (CS), malnourished saline (DS), fenfluramine Control (FC) and malnourished fenfluramine (DF). The encephalum were criossection and processed with immunohistochemical techniques against the enzyme tryptophan hydroxylase. We analyzed the number of neurons immunoreactive for tryptophan hydroxylase and its distribution within the nuclei of Dorsal and median raphe. The malnourished group showed no changes in the quantity and distribution of serotoninergic cells in the raphe nuclei compared to control animals. However, when the system was stimulated with fenfluramine serotonin, we observed an increase in the number of neurons immunoreactive for tryptophan hydroxylase enzyme in the nucleus Dorsal raphe only in undernourished animals. The investigation of the topographic organization of cells in regions marked rostro-caudal axis revealed significant differences between malnourished and nourished bodies, which were not observed in the formation of nuclei as a whole. Thus, we stress the relevance of studies in sub-populations of neurons within the raphe nuclei, showing different susceptibilities to these shares of perinatal malnutrition

ASSUNTO(S)

raphe nuclei anatomia patologica e patologia clinica fenfluramina malnourished desnutriÃÃo â sistema serotoninÃrgico serotonin nÃcleos da rafe rats triptofano hidroxilase tryptophan hydroxylase fenfluramine

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