Detection of Salmonella in raw foods of animal origin using Tecra Salmonella VIA and Tecra Salmonella Unique rapid immunoassays and a cultural procedure / Detecção de Salmonella em alimentos crus de origem animal empregando os imunoensaios rápidos TECRATM Salmonella VIA, TECRATM Salmonella UNIQUE e o método convencional de cultura

AUTOR(ES)
DATA DE PUBLICAÇÃO

2002

RESUMO

The presence of Salmonella in 200 raw food samples of animal origin was investigated by means of rapid immunoassays TECRA™ Salmonella VIA and TECRA™ Salmonella UNIQUE (TECRA Diagnostics, Rosewille, NSW, Australia) and the cultural procedure used routinely in Instituto Adolfo Lutz, Sao Paulo, SP. Forty-five samples (22.5%) were Salmonella positive by at least one of the three methods. The number of positive samples according to the analytical method was 34 (75.6%) for the cultural procedure, 29 (64.4%) for TECRA™ Salmonella VIA and 27 (60.0%) for TECRA™ Salmonella UNIQUE. The cultural method detected four positive samples that both rapid methods were unable to detect. TECRA™ Salmonella UNIQUE detected seven positive samples that were not detected by the two other methods. One sample was positive by the TECRA™ Salmonella VIA exclusively. Considering overall results (positive and negative) McNemars chi square tests indicated that the differences between results given by the rapid immunoassays when compared to those of the cultural method were not significant (p>0.05).

ASSUNTO(S)

food microbiology salmonella (determination) microbiologia de alimentos salmonella (determinação) alimentos de origem animal (microbiologia) food of animal origin

ACESSO AO ARTIGO

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