ComparaÃÃo entre mÃtodos de imobilizaÃÃo de antÃgeno de Trypanosoma cruzi em suporte de ouro

AUTOR(ES)
DATA DE PUBLICAÇÃO

2003

RESUMO

The recombinant Trypanosoma cruzi antigen (CRA~FRA) supplied by FundaÃÃo Biomanguinhos - Rio de Janeiro, Brazil, was attached to gold support using three immobilization methods, such as: physical adsorption, via polyethylenimine and via polyethylenimine plus glutaraldehyde as bi-functional agent. This last method showed best results with increased values of sensitivity measured by Fold Increased (positive and negative OD490 ration). The optimal antigen concentration was established and it was found that the amount of antigen such as 100 μL at 1.0 μg/mL for 9 mm2 gold support area was required. A comparison between this procedure and the ELISA Biomanguinhos-protocol was described. A reliable method of immobilization T. cruzi antigen on gold support was achieved which open new perspectives for the biosensors development

ASSUNTO(S)

adsorÃÃo fÃsica immobilization gold support methods t. cruzi antigen trypanosoma cruzi glutaraldeÃdo bioquimica polietilenoimina

ACESSO AO ARTIGO

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