Clostridium Perfringens Type D
Mostrando 1-12 de 52 artigos, teses e dissertações.
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1. Determination of multidrug resistance mechanisms in Clostridium perfringens type A isolates using RNA sequencing and 2D-electrophoresis
In this study, we screened differentially expressed genes in a multidrug-resistant isolate strain of Clostridium perfringens by RNA sequencing. We also separated and identified differentially expressed proteins (DEPs) in the isolate strain by two-dimensional electrophoresis (2-DE) and mass spectrometry (MS). The RNA sequencing results showed that, compared w
Braz J Med Biol Res. Publicado em: 11/06/2018
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2. Mapping of the continuous epitopes displayed on the Clostridium perfringens type D epsilon-toxin
Abstract The epsilon toxin, produced by Clostridium perfringens, is responsible for enterotoxemia in ruminants and is a potential bioterrorism agent. In the present study, 15 regions of the toxin were recognized by antibodies present in the serum, with different immunodominance scales, and may be antigen determinants that can be used to formulate subunit vac
Braz. J. Microbiol.. Publicado em: 2017-07
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3. Clostridium perfringens isolate typing by multiplex PCR
Clostridium perfringens is an important pathogen that provokes numerous different diseases. This bacterium is classified into five different types, each of which capable of causing a different disease. There are various methods for the bacterial identification, many are labor-intensive, time-consuming, expensive and also present low sensitivity and specifici
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2010
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4. Genotyping of Clostridium perfringens associated with sudden death in cattle
Toxigenic types of Clostridium perfringens are significant causative agents of enteric disease in domestic animals, although type E is presumably rare, appearing as an uncommon cause of enterotoxemia of lambs, calves and rabbits. We report herein the typing of 23 C. perfringens strains, by the polymerase chain reaction (PCR) technique, isolated from small in
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2009
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5. Isolamento e tipifícação genotípica de Clostridium Perfringens em frangos de corte.
Clostridium perfringens is an anaerobic Gram-positive bacterium which causes gaseous gangrene and enterotoxaemias in humans and domestic animals besides being the primary cause of necrotic enteritis in poultry. Clostridium perfringens strains were isolated 171/250 (68.4%) from the intestinal content of broiler chickens sampled in a slaughterhouse in Pará de
Publicado em: 2007
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6. Clostridium perfringens types A and D associated with enterotoxemia in an 18-month-old goat
Postmortem examination of a Boer buck that died peracutely revealed bowel and liver diffusely congested and edematous. Kidney was apparently edematous. Clostridium perfringens type A was isolated from bowel and type D from kidney. Microscopic examination revealed large areas of necrosis in the renal cortex and medulla (pulpy kidney disease), hyperemia and ce
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2007
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7. Enterotoxemia em bovino
This report describes a case of bovine enterotoxaemia in Morro da Garça, Minas Gerais, Brazil. Clostridium perfringens type D was isolated in pure culture and was characterized by biochemical reactions and PCR. By the mouse neutralization test, the presence of epsilon toxin from intestinal content was detected.
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Publicado em: 2006-10
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8. Produção e caracterização de anticorpos monoclonais anti épsilon toxina de Clostridiun perfrigens tipo D
Monoclonal antibodies reactive against épsilon toxin from Clostridium perfringens type D were produced. Myeloma cell lines Sp2/0. Ag14 and P3-X63-Ag8.653 were fused with spleen cells from mice BALB/c immunized with epsilon toxoid. After fusion 59 wells were positive for the Sp2/0. Ag14 cell line and 19 for the P3-X63-Ag8.653, representing 6.68 and 6.59% res
Publicado em: 2006
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9. Detection of alpha, beta and epsilon toxin genes of Clostridium perfringens isolated from cattle?s clinical samples by polimerase chain reaction / Detecção dos genes das toxinas alfa, beta e épsilon de Clostridium perfringens isolados a partir de amostras clínicas de bovinos pela reação em cadeia da polimerase
Clostridium perfringens is an anaerobic micro-organism that is present in the soil and gastrointestinal tract of mammals. It causes food poisoning in humans, enterotoxemic diseases in domestic animals and gas gangrene in both. C. perfringens is classified into five types (A, B, C, D and E) according to the production of four major toxins (alpha, beta, epsilo
Publicado em: 2004
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10. Esterase electrophoresis of Clostridium perfringens bovine strains
Eighty-nine of 144 isolates of Clostridium perfringens obtained from 187 samples of 71 bovine in several Brazilian states were submitted to esterase electrophoresis for typing. Mobilites electrophoresis, as parameter, were settled down by isolates from ATCC pattern of types A, B, C, and D. Of the 89 isolates, 43 (48.3%) were characterized as electrophoretic
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2003
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11. Effect of Clostridium perfringens epsilon toxin on the cardiovascular system of rats.
Pressor activity was demonstrated by the intravenous injection of purified epsilon toxin of Clostridium perfringens type D but not by that of epsilon prototoxin. The activity was completely abolished by C. perfringens type B or D antiserum, but not by type A, C, or E antiserum. The rise in blood pressure caused by the toxin was accompanied by a decrease in t
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12. SEROLOGY OF THE SOLUBLE ANTIGENS OF CLOSTRIDIUM PERFRINGENS TYPES A-F BY AGAR-GEL DIFFUSION
Ellner, Paul D. (University of Vermont, Burlington) and Carolyn D. Bohan. Serology of the soluble antigens of Clostridium perfringens types A–F by agar-gel diffusion. J. Bacteriol. 83:284–296. 1962.—A serological study by agar-gel diffusion of the soluble antigens of 39 strains of the Clostridium perfringens group has shown them to be extremely heterog