Isolamento e tipifícação genotípica de Clostridium Perfringens em frangos de corte.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

Clostridium perfringens is an anaerobic Gram-positive bacterium which causes gaseous gangrene and enterotoxaemias in humans and domestic animals besides being the primary cause of necrotic enteritis in poultry. Clostridium perfringens strains were isolated 171/250 (68.4%) from the intestinal content of broiler chickens sampled in a slaughterhouse in Pará de Minas, MG. Clostridium perfringens were identified according to Gram staining, lecithinase test on agar TSC-egg yolk, haemolysis in desfibrinated horse blood agar and biochemical tests. Clostridium perfringens strains were classified into five toxigenic types (A -E), which were based on the detection of codifying alfa (cpa), beta (cpb), epsilon (etx) e iota (iA) genes, using multiplex PCR assay for genotyping of the principal and lethal toxins, beta2 toxin (cpb2) and enterotoxin (cpe). Clostridium perfringens strains were isolated in 62/125 (49.60%) from jejunum content and 109/125 (87.20%) strains from ileum. From a total of 62 Clostridium perfringens jejunum isolates obtained 42/62 (67.74%) were type A, 1/62 (1.61%) type A with amplification of products for beta2 toxin gene, 0/62 (0%) type B, 17/62 (27.42%) type C and 1/62 (1.61%) type D. A total of 109 ileum Clostridium perfringens isolates were obtained being 62/109 (56.88%) type A, 3/109 (2.75%) type A with amplification of products for beta2 toxin gene, 1/62 (0.92%) type B, 38/109 (34.86%) type C, 1/109 (0.92%) type D. Clostridium perfringens A (60.82%) and Clostridium perfringens C (32.16%) toxigenic types were the most prevalent types in the analyzed parts of the intestinal content of broiler chickens. Five (2.92%) out of a total of 171 Clostridium perfringens strains were not typified. Clostridium perfringens codifying the iota toxin gen and enterotoxin gene were not detected.

ASSUNTO(S)

intestinos doenças teses clostridium perfringens teses frango de corte doenças teses

ACESSO AO ARTIGO

Documentos Relacionados