Yeast Two Hybrid
Mostrando 1-12 de 1385 artigos, teses e dissertações.
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1. Network analysis of ABA-dependent and ABA-independent drought responsive genes in Arabidopsis thaliana
Abstract Drought is one of the most severe abiotic factors restricting plant growth and yield. Numerous genes functioning in drought response are regulated by abscisic acid (ABA) dependent and independent pathways, but knowledge of interplay between the two pathways is still limited. Here, we integrated transcriptome sequencing and network analyses to explor
Genet. Mol. Biol.. Publicado em: 23/07/2018
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2. Identification and characterization of MAGO and Y14 genes in Hevea brasiliensis
Abstract Mago nashi (MAGO) and Y14 proteins are highly conserved among eukaryotes. In this study, we identified two MAGO (designated as HbMAGO1 andHbMAGO2) and two Y14 (designated as HbY14aand HbY14b) genes in the rubber tree (Hevea brasiliensis) genome annotation. Multiple amino acid sequence alignments predicted that HbMAGO and HbY14 proteins are structura
Genet. Mol. Biol.. Publicado em: 2016-03
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3. TNNI3K is a novel mediator of myofilament function and phosphorylates cardiac troponin I
The phosphorylation of cardiac troponin I (cTnI) plays an important role in the contractile dysfunction associated with heart failure. Human cardiac troponin I-interacting kinase (TNNI3K) is a novel cardiac-specific functional kinase that can bind to cTnI in a yeast two-hybrid screen. The purpose of this study was to investigate whether TNNI3K can phosphoryl
Braz J Med Biol Res. Publicado em: 01/02/2013
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4. Caracterização funcional da interação entre as proteínas CTSP-1 e CTCF / Functional characterization of the interaction between the proteins CTSP-1 and CTCF.
Cancer-testis (CT) antigens are immunogenic proteins expressed in gametogenic tissues and in different histological types of tumors, being considered promising candidates for cancer immunotherapy. However, little is known about their role in tumorigenesis. In 2006, we identified CTSP-1 as a novel CT antigen, frequently expressed in different types of tumors.
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 06/12/2011
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5. Estudos bioquímicos, funcionais e estruturais da septina humana SEPT2: fatores que determinam a formação de agregados / Functional and structural studies of human SEPT2: determinant factors triggering the sefl-assembly into amyloid fibrils
As septinas fazem parte de uma família de proteínas de ligação ao nucleotídeo guanina. As septinas têm mostrado ter um papel importante na citocinese e outros processos celulares, incluindo a determinação da polaridade celular e reorganização do citoesqueleto. Todos os membros da família de septinas são compostos por três domínios: um N-termina
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 26/10/2011
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6. Estudos estruturais e funcionais das proteínas cinases humanas Nek1 e Nek6 / Structural and functional studies of Nek1 Nek6 protein kinases
NIMA was identified and functionally characterized in Aspergillus nidulans as a critical Ser/Thr kinase for cell cycle progression. The mammalian Neks (NIMA-related kinases) represent an evolutionarily conserved family of 11 serine/threonine kinases that share 40-45% identity with NIMA N-terminal domain. Neks are associated to cell cyclerelated functions and
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 04/03/2011
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7. Interaction between human cytomegalovirus UL136 protein and ATP1B1 protein
Interplay between the host and human cytomegalovirus (HCMV) has a pivotal role in the outcome of infection. A region (referred to as UL/b’) present in the Toledo strain of HCMV and low passage clinical isolates contains 19 additional genes, which are absent in the highly passaged laboratory strain AD169. Products of the UL/b’ genes may determine the mani
Brazilian Journal of Medical and Biological Research. Publicado em: 2011-12
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8. Analysis of the stress-inducible transcription factor SsNAC23 in sugarcane plants
Stresses such as cold and drought can impair plant yield and induce a highly complex array of responses. Sugarcane (Saccharum spp.) is cultivated in tropical and subtropical areas and is considered a cold-sensitive plant. We previously showed that cold stress induces the expression of several genes in in vitro sugarcane plantlets. Here we characterize one of
Scientia Agricola. Publicado em: 2011-08
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9. Análises moleculares das vias de sinalização de morte celular originadas no retículo endoplasmático / Molecular analyses of cell death pathways signalling originated in the endoplasmic reticulum
Abiotic and biotic stresses are responsible for major losses in agricultural productivity world wise. Therefore, the understanding of mechanisms of plant stress response and adaptation to such conditions should provide the foundation for engineering plant stress tolerance and hence maximizing the yield of crops growing under adverse environmental conditions.
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 11/02/2010
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10. Caracterização funcional e estrutural das septinas humanas 1, 6 e 8 / Functional and structural characterization of human septins 1, 6 and 8
Septins have a central domain which binds GTP (GTPase domain) and two other variable domains at their N- and C-termim Their principal characteristic is the ability to bind to other septins, thereby forming filaments. This is intimately related to their functions in cytokinesis, exocytosis, and apoptosis, among others. They are also directly or indirectly ass
Publicado em: 2010
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11. Neurospora crassa mat A-2 and mat A-3 proteins weakly interact in the yeast two-hybrid system and affect yeast growth
Mating-type genes control the entry into the sexual cycle, mating identity and sexual development in fungi. The mat A-2 and mat A-3 genes, present in the mat A idiomorph of the filamentous fungus Neurospora crassa, are required for post-fertilization functions but are not essential for mating identity. Their putative roles as transcription factors are based
Genetics and Molecular Biology. Publicado em: 2009
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12. Estudos estruturais e funcionais da proteina stanniocalcina-1 humana, um novo marcador de microambiente de leucemia / Structural and functional studies of human protein stanniocalcina-1, a new microenvironmental marker of leukemia
Staniocalcins (STCs) represent a small family of glycoprotein hormones found in all vertebrates composed by STC1 and STC2, which have been initially implicated in calcium homeostasis and also recently implicated in several other processes. Stanniocalcin-1 (STC1), the first member found, was originally discovered in bony fishes and later identified in humans
Publicado em: 2009