Network analysis of ABA-dependent and ABA-independent drought responsive genes in Arabidopsis thaliana
AUTOR(ES)
Liu, Shiwei, Lv, Zongyou, Liu, Yihui, Li, Ling, Zhang, Lida
FONTE
Genet. Mol. Biol.
DATA DE PUBLICAÇÃO
23/07/2018
RESUMO
Abstract Drought is one of the most severe abiotic factors restricting plant growth and yield. Numerous genes functioning in drought response are regulated by abscisic acid (ABA) dependent and independent pathways, but knowledge of interplay between the two pathways is still limited. Here, we integrated transcriptome sequencing and network analyses to explore interplays between ABA-dependent and ABA-independent pathways responding to drought stress in Arabidopsis thaliana. We identified 211 ABA-dependent differentially expressed genes (DEGs) and 1,118 ABA-independent DEGs under drought stress. Functional analysis showed that ABA-dependent DEGs were significantly enriched in expected biological processes in response to water deprivation and ABA stimulus, while ABA-independent DEGs were preferentially enriched in response to jasmonic acid (JA), salicylic acid (SA) and gibberellin (GA) stimuli. We found significantly enriched interactions between ABA-dependent and ABA-independent pathways with 94 genes acting as core interacting components by combining network analyses. A link between ABA and JA signaling mediated through a direct interaction of the ABA responsive elements-binding factor ABF3 with the basic helix-loop-helix transcription factor MYC2 was validated by yeast two-hybrid and bimolecular fluorescence complementation (BiFC) assays. Our study provides a systematic view of the interplay between ABA-dependent and ABA-independent pathways in response to drought stress.
Documentos Relacionados
- Effects of molybdenum on expression of cold-responsive genes in abscisic acid (ABA)-dependent and ABA-independent pathways in winter wheat under low-temperature stress
- Biochemical characterization of the aba2 and aba3 mutants in Arabidopsis thaliana.
- Genes controlling fertilization-independent seed development in Arabidopsis thaliana
- Ammopiptanthus mongolicus stress-responsive NAC gene enhances the tolerance of transgenic Arabidopsis thaliana to drought and cold stresses
- Drought Rhizogenesis in Arabidopsis thaliana (Differential Responses of Hormonal Mutants).