Yeast Two Hybrid
Mostrando 13-24 de 1385 artigos, teses e dissertações.
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13. Characterization of the type 2A phosphatase regulatory protein, TIPRL and alpha4 / Caracterização das proteinas TIPRL e alfa4, reguladores de fosfatases 2A
Cells respond constantly to a variety of stimuli, which are interpreted and integrated through signaling networks, giving rise to biological responses. Defects in this circuitry are a cause of many diseases, including cancer. Protein phosphatases are enzymes which remove phosphate groups from kinase substrates, relying mainly on regulatory subunits for their
Publicado em: 2009
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14. Estudos das interações da septina 4 humana / Study of Human Septin 4 interactions
Septinas são proteínas ligantes a GTP encontradas desde fungos até metazoários. A primeira função identificada para septinas foi o seu papel central na organização e dinâmica do septo de divisão de leveduras. Uma das características marcantes é que septinas se organizam em heterofilamentos de 7 a 9 nm de espessura que foram purificados de diverso
Publicado em: 2009
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15. Genes diferencialmente expressos em Paracoccidioides brasiliensis
Paracoccidioides brasiliensis is a thermally dimorphic fungus causing paracoccidioidomycosis, an endemic disease widespread in Latin America. The dimorphic transition from the mycelia (22 C) to the yeast (36 C) form is induced by a shift from the environmental temperature to that of the mammalian host. Genes that are differentially expressed in phases of P.
Publicado em: 2008
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16. Functional analysis of the human protein encoded by the new interferon stimulated gene ISG95 / Analise funcional da proteina humana codificada pelol novo gene de resposta a interferon ISG95
A resposta individual das células está na base da resistência do organismo à infecção viral. O principal mecanismo de resistência envolve a participação de inúmeros genes da via de sinalização dos interferons. Vários estudos vêm sendo conduzidos em larga escala para identificar genes que respondem aos mais variados tratamentos, assim como clust
Publicado em: 2008
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17. Identificação e estudo funcional de genes associados com doenças neurológicas / Identification an functional estudy of genes associated with neurological diseases
In this work we have used different approaches to the study of genes associated with CNS development and function as well as with neurological diseases: 1) one study involved the identification of the allele associated with an X-linked recessive sindromic form of mental retardation, Snyder-Robinson syndrome, in a Brazilian family. Using genetic linkage analy
Publicado em: 2008
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18. Estudos funcionais e estruturais da proteina humana hnRNP Q/NSAP1 / Funcional and structural studies of human protein hnRNPQ
The members of the hnRNPs family (heterogenous nuclear ribonuclein proteins) play important roles in gene expression control and mRNAs metabolism. The proteins hnRNPD (AUF1) and hnRNPQ (NSAP1) were the main targets of this study. AUF1 has two RNA recognition motifs (RRM) and participates in the process of destabilization of a class of mRNAs that contain AU-r
Publicado em: 2008
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19. Obtaining a fluorescent and resistant hybrid yeast to the nystatin / Obtenção de levedura híbrida fluorescente e resistente a nistatina
Vats contamination by wild yeasts can harm the efficiency and the productivity of ethanol industrial production and there are not efficient methods to control this type of contamination. Easiness, speed and the low cost would be desirable characteristics for the control of yeasts that eventually contaminate the fermentation process. With this aim, TAVARES (1
Publicado em: 2007
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20. Identificação de interações proteína-proteína entre NS5 do vírus da febre amarela e proteínas celulares.
Yellow fever is an infectious disease caused by the yellow fever virus (YFV), a Flavivirus transmitted to humans by Aedes aegypti mosquitoes. Despite the existence of the yellow fever vaccine, the disease is endemic in South America and Africa, causing public health problems such as dispersed outbreaks, epidemics with variable impact and the risk of re-emerg
Publicado em: 2007
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21. Study of the function of the protein Nop53p in Saccharomyces cerevisiae / Caracterização da função da proteína Nop53p de Saccharomyces cerevisiae
In eukaryotes, the rRNA processing depends on several factors, such as, endonucleases, exonucleases, RNA helicases, rRNA modifying enzymes and components of the snoRNPs. With the purpose of characterizing new proteins involved in pre-rRNA processing, Nop53p was identified interacting with the nucleolar protein Nop17p in a two hybrid assay. The conditional ye
Publicado em: 2007
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22. Estudo funcional e estrutural de Nip7p, uma proteina conservada envolvida na sintese de ribossomos / Functional and structural analysis of Nip7p, a conserved protein involved in ribosome biogenesis
A síntese de ribossomos é um processo conservado em eucariotos e se inicia com a transcrição dos rRNAs no nucléolo. Mais de 170 fatores atuam de forma transitória no processamento dos precursores para gerar os rRNAs maduros que formarão as subunidades ribossomais no citoplasma. Entre as proteínas envolvidas na síntese de ribossomos está a Nip7p, um
Publicado em: 2007
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23. Analysis of the interaction between human kidney anion exchanger 1 and kanadaptin using yeast two-hybrid systems
Kidney anion exchanger adaptor protein (Kanadaptin) is a protein which interacts with the cytoplasmic N-terminal domain of kidney anion exchanger 1 (kAE1) and was first detected in mice using the yeast two-hybrid system and was also found to co-localize with kAE1 in rabbit a-intercalated cells. Impaired trafficking of human kAE1 can result in the kidney dise
Genetics and Molecular Biology. Publicado em: 2006
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24. PROTEIN-PROTEIN INTERACTION ANALYSIS OF THE DEFENSIN PSD1 FROM PISUM SATIVUM WITH NEUROSPORA CRASSA PROTEINS / ANÁLISE DA INTERAÇÃO PROTÉICA DA DEFENSINA PSD1 DE PISUM SATIVUM COM PROTEÍNAS DO FUNGO NEUROSPORA CRASSA
Plant defensins, innate components of the plant immune system, are cationic, antifungal peptides, with a cysteine- rich primary structure. Evidence from the literature demonstrated that fungus membrane patches containing complex sphingolipids, mannosyldiinositolphosphorylceramide and glucosylceramides, are selective binding sites for the plant defensins isol
Publicado em: 2006