Turbidimetric Assay
Mostrando 1-12 de 45 artigos, teses e dissertações.
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1. Comparison of two rotary systems in bacteria/lps removal from endodontic infections: randomized clinical trial
Abstract: This clinical study compared the effectiveness of two rotary systems: HyFlex CM (Coltene-Whaledent, Altstetten, Switzerland) and ProTaper Next (Dentsply Sirona, Ballaigues, Switzerland) on the removal of cultivable bacteria and endotoxins from primarily infected root canals. This study was designed as a randomized single-blinded, 2-arm, clinical tr
Braz. oral res.. Publicado em: 16/05/2019
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2. Development and validation of a rapid turbidimetric assay to determine the potency of norfloxacin in tablets
O norfloxacino foi a primeira fluorquinolona (e mais utilizada) disponível no mercado. Este trabalho divulga um novo desenvolvimento e validação de um método turbidimétrico simples, sensível, preciso e reprodutível para a quantificação de norfloxacino em comprimidos em apenas 4 horas. O bioensaio é baseado no efeito inibitório de norfloxacino sobr
Braz. J. Pharm. Sci.. Publicado em: 2015-09
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3. Monitoring the effectiveness of root canal procedures on endotoxin levels found in teeth with chronic apical periodontitis
Objective: The aim of this study was to monitor the effectiveness of root canal procedures by using different irrigants and intracanal medication on endotoxin levels found in root canals of teeth with chronic apical periodontitis. Material and Methods: Thirty root canals of teeth with pulpal necrosis associated with periapical lesions were selected and ra
J. Appl. Oral Sci.. Publicado em: 29/07/2014
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4. Determinação de heparina fracionada em preparações farmacêuticas utilizando turbidimetria
A turbidimetric method has been used for quantification of fractionated heparin (FH) in pharmaceutical dosage. The UV detection at two wavelengths (290 and 500 nm) showed a significant increase in sensitivity of the method, specificity, and linearity to range 5.0-50.0 µg mL-1 and 50.0-200.0 µg mL-1, respectively (r < 0.99). At both wavelengths, the method
Química Nova. Publicado em: 2011
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5. Sistemas de análises químicas em fluxo explorando mecanismos de re-alimentação, calibração multivariada e outras abordagens para melhoria em desempenho / Flow systems exploiting feed-back mechanisms, multivariate calibration and other strategies for improving the analytical performance
Studies focusing on performance of intelligent analytical flow systems, association with multivariate calibration or use of nanocrystals as sensitizers were carried out. Moreover, improvements related to modifications in flow pattern were evaluated with emphasis to in-line dialysis. To this end, a strategy for implementing simultaneous determinations of iron
Publicado em: 2010
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6. Doseamento microbiológico de apramicina - desenvolvimento e validação de método empregando leitura cinética em microplacas / Microbial assay of apramycin - delevopment and validation of assay using kinetic-reading microplate system
O princípio do ensaio turbidimétrico é simples: a solução-teste é adicionada à suspensão do microrganismo-teste em meio de cultura, a mistura é incubada em condições adequadas e o crescimento microbiano é medido através da leitura fotométrica. O emprego de método de microplacas com leitura cínética para a dosagem de antibióticos é de inter
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 16/10/2009
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7. A modified method for the turbidimetric assay of nisin
Nisina é um peptídeo antibacteriano produzido por Lactococcus lactis subsp lactis que apresenta um grande espectro antimicrobiano para microrganismos gram-positivos incluindo esporos bacterianos. Alguns métodos para deteminação da atividade de nisina foram definidos. Neste trabalho foi proposto uma modificação no método para determinação de nisina
Brazilian Archives of Biology and Technology. Publicado em: 2003-06
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8. New turbidimetric assay for quantitation of viable bacterial densities.
A turbidimetric assay was developed and validated against Escherichia coli for the quantitation of viable bacterial densities. The Abbott MS-2 research system was employed for continuous 5-min measurements of optical density. A linear standard curve was obtained by regressing the initial bacterial density (log CFU per milliliter) against the time required fo
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9. Turbidimetric Assay of Staphylococcal Nuclease
A simplified turbidimetric procedure was developed to assay staphylococcal nuclease activity. The ease of performance and sensitivity to nanogram quantities enhance the utilization of the method for the quantitative or qualitative estimation of the enzyme. Unlike plating methods, the turbidimetric procedure affords the differentiation between heat-stable and
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10. Effects of amines and polyamines on turbidimetric and lysoplate assays for lysozyme.
The lysoplate and turbidimetric assays have often been used to measure lysozyme in biological fluids, such as blood and saliva. When the same purified lysozyme standard is used in both assays, results obtained with the lysoplate assay are much higher than those obtained for the same sample in the turbidimetric assay. It is likely, therefore, that other compo
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11. Semiautomated Turbidimetric Microbiological Assay for Determination of Cefazolin
The Autoturb System, a semiautomated system for photometric bioassay, was used to determine cefazolin content. Suitable conditions for the assay using Streptococcus faecalis ATCC 10541 as the indicator organism included a medium pH of 6.0 to 7.0 and an incubation time of 3 to 3.5 h at 36 C. Multiple independent assays of samples from a common batch showed th
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12. Turbidimetric Bioassay for Carbenicillin
A quantitative turbidimetric bioassay has been developed for carbenicillin with the use of a strain of Escherichia coli. The assay is relatively specific for carbenicillin and is not affected by 10% (wt/wt) benzylpenicillin. It has been demonstrated to be more precise than the plate diffusion assay. Since it is readily automated, it is also faster and less e