Turbidimetric Assay
Mostrando 13-24 de 45 artigos, teses e dissertações.
-
13. Factors influencing measurement of human salivary lysozyme in lysoplate and turbidimetric assays.
The use of different assay conditions has complicated the evaluation of studies relating salivary lysozyme levels to oral or systemic disease. The purpose of this study was to compare values obtained for lysozyme activity in mixed saliva of 104 healthy subjects by using two assay techniques and four variations in sample preparation. Lysozyme activity was ass
-
14. A TURBIDIMETRIC METHOD FOR THE ASSAY OF ANTIBIOTICS
-
15. Development of an enzyme-linked immunosorbent assay for determination of lysozyme in human parotid and submandibular-sublingual salivas.
The specificity of lysozyme determinations in human parotid and submandibular-sublingual salivas of two subjects was assessed by comparison of lysozyme concentrations in native acidified salivas with purified enzyme obtained by immunoadsorbent fractionation of the salivas. Lysozyme concentrations were measured by the turbidimetric catalytic method and by a n
-
16. Comparative Study of Responses to Neomycins B and C by Microbiological and Gas-Liquid Chromatographic Assay Methods
The relative responses of neomycins B and C have been determined by a microbiological agar-diffusion method, a turbidimetric method, and by a recently developed gas-liquid-chromatographic (GLC) method capable of separating the neomycin isomers. The ratios of response of neomycin C to neomycin B by the individual methods were as follows: agar-diffusion method
-
17. Automated Turbidimetric Bioassay Readout Instrument Using a Multiple Flow-Cell System
An instrument has been developed which permits the automatic quantitation of the turbidities of microbiological assay samples. Assay tubes were fed to the instrument at the end of the incubation period. The turbidity readings were automatically converted to digital data which were printed on International Business Machines (IBM) cards and from which potencie
-
18. Dose Response Curve Linearization and Computer Potency Calculation of Turbidimetric Microbiological Vitamin Assays
The dose response curves of various turbidimetric microbiological assays, including vitamin B12, Ca pantothenate, and pyridoxine (vitamin B6), were linear with logarithmic transformation of the responses by use of the equation derived, ln[T(x) - T(∞)] = ln A - Bx. A Fortran computer program which used the slope ratio potency calculation was written. The as
-
19. Quantitative assay of soluble beta-hemolytic streptococcal antigens via an immunochemical turbidimetric method with a spectrophotometer.
Soluble, group A, B, C, and G beta-hemolytic streptococcal antigens were successfully identified in a prototype spectrophotometric system by an immunochemical turbidimetric assay. Any spectrophotometic system which can take a zero reading followed by a second reading 2 or more min later can be used for the assay. Maximum absorbance was obtained near a wavele
-
20. Comparison of an immunochemical assay for plasma fibrinogen and a turbidimetric thrombin clotting technique to discriminate hyperlipidaemic patients from healthy controls.
Plasma samples from patients attending a lipid clinic (n = 14) and healthy control subjects (n = 21) were assayed for fibrinogen using an immunochemical method (radial immunodiffusion) and a turbidimetric assay based on the thrombin clotting technique. The patients had significantly higher plasma fibrinogen concentrations than controls by both methods, but t
-
21. Turbidimetric Changes Accompanying the Interaction of Endotoxin and the α Chains of Human Hemoglobin1
Setlow, Peter (Brandeis University, Waltham, Mass.), Lawrence Levine, and Helen Van Vunakis. Turbidimetric changes accompanying the interaction of endotoxin and the α chains of human hemoglobin. J. Bacteriol. 89:1237–1243. 1965.—With the use of a turbidimetric assay, the effects of pH, ionic strength, anions and cations, and varying concentrations of re
-
22. Rapid Microbiological Assay for Chlorhydroxyquinoline That Uses a Cryogenically Stored Inoculum
A rapid microbiological assay for chlorhydroxyquinoline is described. It is a turbidimetric procedure that uses Streptococcus faecalis as the test organism. Results are available within 5 h. Data are presented to show the advantages of using a cryogenically stored inoculum over an inoculum prepared on a daily basis.
-
23. Stable Defined Substrate for Turbidimetric Assay of Endoxylanases
A stable xylan suspension was prepared and characterized. Hydrolysis of the particles converts them into soluble fragments, thereby lowering the turbidity of the suspension. The small volume of the assay mixture, the short incubation time required, and the simplicity of the procedure permit the rapid analysis of many samples. Furthermore, the procedure can b
-
24. Semiautomated Turbidimetric Bioassay for the Ionophore A23187
The inhibitory effect of A23187 against Staphylococcus aureus was used to quantitate the activity of this ionophore in fermentation samples and in isolation and crystallization samples. The assay was shown to be rapid and reproducible.