Triple Helix
Mostrando 13-24 de 281 artigos, teses e dissertações.
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13. University-industry technological cooperation for energy efficiency: a case study
The central aim of this article is to demonstrate the development and characteristics of an inter-institutional relationship with a view to perfecting existing technology in reducing the consumption of energy in household refrigerators and also to show the adequacy of the case studied for the most recent preconceived ideas concerning the development of Etzko
BAR - Brazilian Administration Review. Publicado em: 2006-06
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14. A tríplice hélice e o desenvolvimento do setor de tecnologia da informação no Distrito Federal
A crescente interação entre os segmentos universidade, governo e empresa indica a tendência mundial de alianças e parcerias como parte do processo de desenvolvimento da sociedade. Esta pesquisa estuda o setor de Tecnologia da Informação (TI) no Distrito Federal e a sua relação com a Universidade de Brasília, o Governo Federal, o Governo do Distrito
Publicado em: 2006
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15. O papel do CITS1 na política de desenvolvimento tecnológico no Paraná
Analisam-se as implicações da produção de software no Paraná. Como produção de bem intangível, há contribuições para a expansão do Sistema Setorial de Inovação. O foco da análise é o Centro Internacional de Tecnologia de Software e sua trajetória natural em direção à capacitação para a inovação radical de forma híbrida. Discute-se a
Production. Publicado em: 2005-12
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16. Sistema de inovaÃÃo baseado em conhecimento e o modelo trÃplice hÃlice : a universidade na construÃÃo da vantagem competitiva do setor de tecnologia da informaÃÃo de Pernambuco
Conhecimento, tanto aquele atribuÃdo ao senso comum como aquele identificado por tÃcnico ou cientÃfico, Ã indispensÃvel para que os indivÃduos estabeleÃam relaÃÃes, integrando-se em um contexto social contemporÃneo cada vez mais dinÃmico e complexo. Temas recorrentes, vinculados ao desenvolvimento socioeconÃmico de um paÃs, tambÃm estÃo intima
Publicado em: 2005
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17. Obtenção e caracterização de blendas colágeno-quitosana
Biodegradable polymer blends were obtained using collagen and chitosan. Membranes of collagen and chitosan in different proportions (3:1, 1:1 and 1:3) were prepared by mixing their acetate solutions (pH 3.5) at room temperature. The blends were characterized by differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), Fourier Transform infra
Química Nova. Publicado em: 2002-11
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18. Poly(rA) binds poly(rG).poly(rC) to form a triple helix.
Poly(rA) binds poly(rG).poly(rC) to form a triple helix. Evidence for this structure includes ultraviolet absorbance mixing curves and melting curves, and circular dichroism spectroscopy. The formation of the triple helix depends on the length of the poly(rC) strand. Triple helix forms when the average length is around 100 nucleotides but does not form when
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19. Triple helix formation inhibits transcription elongation in vitro.
We have identified a 15-nucleotide site within a G-free transcription cassette that forms triple helix with sequence-specific oligodeoxyribonucleotides. When oligodeoxynucleotides were added to template DNA prior to in vitro transcription, a significant fraction of transcripts were truncated at a site corresponding to the region of triple helix formation. Ki
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20. Repair of triple helix directed psoralen adducts in human cells.
Triple helix forming oligonucleotides can direct DNA damaging agents at specific sites in an intact double helix. In our study, triple helix formation was demonstrated in a SV40 based shuttle vector treated with psoralen linked to a 22-mer purine rich oligonucleotide. UVA irradiation caused a covalent linkage of the oligonucleotide through the psoralen to th
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21. A directional nucleation-zipping mechanism for triple helix formation
A detailed kinetic study of triple helix formation was performed by surface plasmon resonance. Three systems were investigated involving 15mer pyrimidine oligonucleotides as third strands. Rate constants and activation energies were validated by comparison with thermodynamic values calculated from UV-melting analysis. Replacement of a T·A base pair by a C·
Oxford University Press.
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22. Effect of a 5'-phosphate on the stability of triple helix.
An effect of 5'-phosphorylation on the stability of triple helical DNA containing pyrimidine:purine:pyrimidine strands has been demonstrated by both gel electrophoresis and UV melting. A 5'-phosphate on the purine-rich middle strand of a triple helix lowers the stability of triple helix formation by approximately 1 kcal/mol at 25 degrees C. The middle strand
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23. Relief of triple-helix-mediated promoter inhibition by elongating RNA polymerases.
We have characterized triple-helix-mediated inhibition of an artificial bacteriophage promoter with respect to relief of inhibition by incoming RNA polymerases that initiate upstream or downstream from the operator sequence. Whereas oligonucleotide-directed triple-helix formation inhibits the test promoter, promoter activity is restored when the triple-helic
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24. Intercalation of ethidium bromide into a triple-stranded oligonucleotide.
We have examined the ability of a cationic planar chromophore, ethidium bromide, to intercalate into a short, defined triple helix. Using UV absorption, fluorescence spectroscopy and a gel retardation assay we demonstrate that ethidium bromide is able to bind to a triple helix with a lower affinity than to the corresponding duplex. Energy transfer from base