Restriction Mapping Methods
Mostrando 1-12 de 53 artigos, teses e dissertações.
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1. "Contribuição ao estudo da influência da radiação ionizante pré-operatória sobre a marcação do linfonodo sentinela do reto com azul patente: estudo experimental em ratos" / Contribution to the study of the influence of preoperative ionizing radiation on the identification of the sentinel lymph node with patent blue : an experimental study in rats
Previous ionizing radiation induces actinic alterations in peritumoral tissues and thus might influence the localization of the sentinel lymph node. The present study developed an experimental model for the localization of the sentinel lymph node of the rectum of the rat and for the definition of the dose of radiation (calibration curve). The objective was t
Publicado em: 2006
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2. Desempenho de crianças em testes sensoriais discriminativos e afetivos com escalas hibridas ilustradas. / The performance of children in discriminative and affective sensory tests with illustrated hybrid scales.
In both developed and emerging countries, children represent a significant part of the consumer market, a market which, due to globalization, has become extremely competitive and avid for novelties. As the number of products destined for the child food market increases and the role of the child in the decision to buy also increases, sensory evaluations carri
Publicado em: 2006
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3. Molecular genetics of herpes simplex virus. III. Fine mapping of a genetic locus determining resistance to phosphonoacetate by two methods of marker transfer.
We have transferred a genetic locus determining resistance to phosphonoacetic acid (PAAr) from one herpes simplex viral genome to another by two methods of marker transfer. One method requires recombination between an intact DNA molecule and a restriction endonuclease DNA fragment, whereas the other requires repair of a partial heteroduplex formed between th
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4. Rapid physical mapping of the Mycoplasma mobile genome by two-dimensional field inversion gel electrophoresis techniques.
A macrorestriction map of the 780 kbp Mycoplasma mobile (ATCC 43663) genome was constructed. Linking fragments were identified on two-dimensional pulsed-field electrophoresis gels. Either complete double restriction digests or partial and complete single digests were separated in the first and second dimension, respectively. 19 restriction sites of four enzy
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5. Cosmid vectors for rapid genomic walking, restriction mapping, and gene transfer.
We have designed cosmid vectors for rapid genomic "walking" and restriction mapping. These vectors contain the transcription promoters from either bacteriophage SP6, T7, or T3 flanking a unique BamHI cloning site. Mammalian expression modules encoding the dominant marker neomycin phosphotransferase or the amplifiable dihydrofolate reductase gene expressed fr
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6. Strategies for studying heterogeneous genetic traits in humans by using a linkage map of restriction fragment length polymorphisms.
Simple single-gene disorders in humans can be genetically mapped by using traditional methods of linkage analysis and increasingly abundant restriction fragment length polymorphisms (RFLPs). Many human diseases and traits, however, can be expected to be genetically heterogeneous (i.e., caused by any one of several genes), and traditional linkage analysis is
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7. Mapping Mendelian Factors Underlying Quantitative Traits Using RFLP Linkage Maps
The advent of complete genetic linkage maps consisting of codominant DNA markers [typically restriction fragment length polymorphisms (RFLPs)] has stimulated interest in the systematic genetic dissection of discrete Mendelian factors underlying quantitative traits in experimental organisms. We describe here a set of analytical methods that modify and extend
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8. Genomic organization of the mouse T-cell receptor beta-chain gene family.
We have combined three different methods, deletion mapping of T-cell lines, field-inversion gel electrophoresis, and the restriction mapping of a cosmid clone, to construct a physical map of the murine T-cell receptor beta-chain gene family. We have mapped 19 variable (V beta) gene segments and the two clusters of diversity (D beta) and joining (J beta) gene
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9. Mapping sequenced E.coli genes by computer: software, strategies and examples.
Methods are presented for organizing and integrating DNA sequence data, restriction maps, and genetic maps for the same organism but from a variety of sources (databases, publications, personal communications). Proper software tools are essential for successful organization of such diverse data into an ordered, cohesive body of information, and a suite of no
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10. Physical mapping of BK virus DNA with SacI, MboII, and AluI restriction endonucleases.
A new restriction endonuclease, SacI from Streptomyces achromogenes cleaves BK virus (strain MM) DNA into 3 fragments, whereas MboII from Moraxella bovis and AluI from Arthrobacter luteus give 22 and 30 fragments, respectively. All these specific DNA fragments were ordered and mapped on the viral genome by two methods first, by the reciprocal digestion metho
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11. Restriction mapping of the genome of the protozoan parasite Theileria parva.
We have used a modified linking clone strategy and pulsed-field gel electrophoresis to derive a map of the 29 Sfi I and 4 Not I sites in the 10 million base pair genome of the protozoan parasite Theileria parva. This was achieved in the absence of classical genetic information. The map reveals four chromosomes. Several genes, including those for parasite ant
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12. A new approach to genome mapping and sequencing: slalom libraries
We describe here an efficient strategy for simultaneous genome mapping and sequencing. The approach is based on physically oriented, overlapping restriction fragment libraries called slalom libraries. Slalom libraries combine features of general genomic, jumping and linking libraries. Slalom libraries can be adapted to different applications and two main typ
Oxford University Press.