A new approach to genome mapping and sequencing: slalom libraries

AUTOR(ES)

Zabarovska, Veronika I.

FONTE

Oxford University Press

RESUMO

We describe here an efficient strategy for simultaneous genome mapping and sequencing. The approach is based on physically oriented, overlapping restriction fragment libraries called slalom libraries. Slalom libraries combine features of general genomic, jumping and linking libraries. Slalom libraries can be adapted to different applications and two main types of slalom libraries are described in detail. This approach was used to map and sequence (with ∼46% coverage) two human P1-derived artificial chromosome (PAC) clones, each of ∼100 kb. This model experiment demonstrates the feasibility of the approach and shows that the efficiency (cost-effectiveness and speed) of existing mapping/sequencing methods could be improved at least 5–10-fold. Furthermore, since the efficiency of contig assembly in the slalom approach is virtually independent of length of sequence reads, even short sequences produced by rapid, high throughput sequencing techniques would suffice to complete a physical map and a sequence scan of a small genome.

Documentos Relacionados

• Subtraction hybridisation and shot-gun sequencing: a new approach to identify symbiotic loci.
• Cancer genome sequencing: a review
• Polymorphism Ratio Sequencing: A New Approach for Single Nucleotide Polymorphism Discovery and Genotyping
• Genome Sequencing: Using Models to Predict Who's Next
• PCR-enzyme-linked immunosorbent assay and partial rRNA gene sequencing: a rational approach to identifying mycobacteria.