Potexvirus
Mostrando 1-12 de 23 artigos, teses e dissertações.
-
1. Detecção do Cassava common mosaic virus por immunocaptura-RT-PCR em mandioca obtida por cultura de meristemas no estado do Paraná
Em um levantamento conduzido na região noroeste do Paraná, principal região produtora de mandioca do estado, constatou-se que o CsCMV está amplamente disseminado, infectando mais de 90% das plantas de todas as cultivares. Um isolado do CsCMV foi purificado para a produção de um anti-soro policlonal de alto título (1/1000), o qual foi utilizado na inde
Tropical Plant Pathology. Publicado em: 2011-10
-
2. Identificação e caracterização de um isolado de Hydrangea ringspot virus em hortênsia no Estado de São Paulo
Plantas de hortênsia apresentando folhas com anéis cloróticos e necróticos, provenientes de Arujá, estado de São Paulo foram analisadas para identificação da espécie viral. Partículas alongadas filamentosas, medindo cerca de 490 nm, foram observadas ao microscópio eletrônico de transmissão. Oligonucleotídeos para Hydrangea ringspot virus (HdRSV
Summa Phytopathologica. Publicado em: 2011-06
-
3. Development of a fitosanity program for Potyvirus and Potexvirus / Desenvolvimento de um programa de fitossanidade para Potyvirus e Potexvirus
The potato culture (Solanum tuberosum L.) represents the fourth major vegetal production in the world, reaching productivity indexes which overcome that of cerals in 5 times. Potato crop may be affected by several disease. Actually, some references point out near 40 in a total of 70 disorders are caused by viral agents. Among the most important viruses of po
Publicado em: 2009
-
4. Efeito do Potato virus X no conteúdo de fenóis totais e alcalóides em folhas de Datura stramonium
O presente trabalho relata resultados sobre a ação do Potato virus X (PVX) no conteúdo de fenóis totais e alcalóides em folhas de Datura stramonium. Uma diminuição significativa no conteúdo dessas substâncias foi observada nas folhas inoculadas com o PVX (X-I). Entretanto, houve um aumento na porcentagem de fenóis nas folhas friccionadas com tampã
Summa Phytopathologica. Publicado em: 2008-02
-
5. Generation of Subgenomic RNA Directed by a Satellite RNA Associated with Bamboo Mosaic Potexvirus: Analyses of Potexvirus Subgenomic RNA Promoter
Satellite RNA of bamboo mosaic potexvirus (satBaMV), a single-stranded positive-sense RNA encoding a nonstructural protein of 20 kDa (P20), depends on bamboo mosaic potexvirus (BaMV) for replication and encapsidation. A full-length cDNA clone of satBaMV was used to examine the sequences required for the synthesis of potexvirus subgenomic RNAs (sgRNAs). Subge
American Society for Microbiology.
-
6. Influence of White Clover Mosaic Potexvirus Infection on the Endogenous Cytokinin Content of Bean
The cytokinin content in the primary leaves of bean (Phaseolus vulgaris) was monitored for 10 d after inoculation with white clover mosaic potexvirus. The cytokinins were isolated, purified, separated by high-performance liquid chromatography, and quantified by radioimmunoassay. The cytokinins detected at the time of inoculation (d 0) were: (a) the free base
American Society of Plant Physiologists.
-
7. The complete nucleotide sequence of the potexvirus white clover mosaic virus.
The complete nucleotide sequence (5845 nucleotides) of the genomic RNA of the potexvirus white clover mosaic virus (WC1MV) has been determined from a set of overlapping cDNA clones. Forty of the most 5'-terminal nucleotides of WC1MV showed homology to the 5' sequences of other potexviruses. The genome contained five open reading frames which coded for protei
-
8. The open reading frame of bamboo mosaic potexvirus satellite RNA is not essential for its replication and can be replaced with a bacterial gene.
A satellite RNA of 836 nt depends on the bamboo mosaic potexvirus (BaMV) for its replication and encapsulation. The BaMV satellite RNA (satBaMV) contains a single open reading frame encoding a 20-kDa nonstructural protein. A full-length infectious cDNA clone has been generated downstream of the T7 RNA polymerase promoter. To investigate the role of the 20-kD
-
9. Bamboo Mosaic Potexvirus Satellite RNA (satBaMV RNA)-Encoded P20 Protein Preferentially Binds to satBaMV RNA
A satellite RNA of 836 nucleotides [excluding the poly(A) tail] depends on the bamboo mosaic potexvirus (BaMV) for its replication and encapsidation. The BaMV satellite RNA (satBaMV) contains a single open reading frame encoding a 20-kDa nonstructural protein (P20). The P20 protein with eight histidine residues at the C terminus was overexpressed in Escheric
American Society for Microbiology.
-
10. Sequences at the 3′ Untranslated Region of Bamboo Mosaic Potexvirus RNA Interact with the Viral RNA-Dependent RNA Polymerase
The 3′ untranslated region (UTR) of bamboo mosaic potexvirus (BaMV) genomic RNA was found to fold into a series of stem-loop structures including a pseudoknot structure. These structures were demonstrated to be important for viral RNA replication and were believed to be recognized by the replicase (C.-P. Cheng and C.-H. Tsai, J. Mol. Biol. 288:555–565, 1
American Society for Microbiology.
-
11. Sufficient Length of a Poly(A) Tail for the Formation of a Potential Pseudoknot Is Required for Efficient Replication of Bamboo Mosaic Potexvirus RNA
RNAs transcribed from a full-length infectious cDNA clone of the bamboo mosaic potexvirus (strain O) genome, pBaMV-O, were infectious to Nicotiana benthamiana plants. Mutant genomes in which the poly(A) tail is absent or replaced by a 3′ tRNA-like structure from turnip yellow mosaic virus RNA failed to amplify detectably in N. benthamiana protoplasts. No a
American Society for Microbiology.
-
12. The Synthesis of Minus-Strand RNA of Bamboo Mosaic Potexvirus Initiates from Multiple Sites within the Poly(A) Tail
The 3′ terminus of the bamboo mosaic potexvirus (BaMV) contains a poly(A) tail, the 5′ portion of which participates in the formation of an RNA pseudoknot required for BaMV RNA replication. Recombinant RNA-dependent RNA polymerase (RdRp) of BaMV binds to the pseudoknot poly(A) tail in gel mobility shift assays (C.-Y. Huang, Y.-L. Huang, M. Meng, Y.-H. Hs
American Society for Microbiology.