Development of a fitosanity program for Potyvirus and Potexvirus / Desenvolvimento de um programa de fitossanidade para Potyvirus e Potexvirus

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

The potato culture (Solanum tuberosum L.) represents the fourth major vegetal production in the world, reaching productivity indexes which overcome that of cerals in 5 times. Potato crop may be affected by several disease. Actually, some references point out near 40 in a total of 70 disorders are caused by viral agents. Among the most important viruses of potato, virus X and Y usually cause major concern, due to their harmfull effects in productivity and comercialization of potato. Nowadays, the viral infecctions have found their way into Brazil´s potato production reagions in a very fast maner. The state of São Paulo is the most afeccted. Althought potato crop is among the stocks 10 major crops in the country, its production relies on virus-free imported potato-seeds to mantain its production. This is not only responsable by a considerable part of the production costs, but also represents a dangerous entrance door for a number of exotic pathogens inexistent in our territory. Thus, a fitosanity control of imported seed-potato lots, which enter Brazil via Santos harbor in the State of São Paulo, has fundamental importance. The usage of imunodiagnosis like ELISA, althought being efficient and widely applied, results in an additional cost due to the dependency of Brazil on the importation of policlonal antisera and monoclonal antibodys necessaries for the proper detection of the most important virus afeccting the national potato plantations. It has becoming a must for the seed-potato production a program in Brazil to have our independence of the diagnoses antiserum reagents for ELISA to at least the most important seed-potato viruses. The independence on importation of these biotecnological products and the modernization of imunodiagnosis by applying molecular techniques with specifics primers and like IC-RT-PCR (ImmoCapture - Reverse Transcriptase - Polimerase Chain Reaction), which makes possible to detect the major potato´s virus in a fast, efficient and cheap way in order to screen the sanity of the material produced in the country, leading to a increase in production and stating the quality of it. This work isolated and purified the virus X and Y by biological indexing and extraction protocols. Therefore, it was possible to produce and to characterize the polyclonals antiserum to PVX and to PVY, with great specificity and power of detection. After these steps, it was made specifics primers and the development of molecular detection techniques based on extraction of genetic material from infected leaves and PCR with the primers. The expected fragments was obtained, concluding with successful the establishment of the test. Finally, there was the attempt to establish the IC-RT-PCR, however the small size of viral particles prevented the test, but the implementation of detection by ELISA and molecular separately was successful, add new national technologies allowing and contributing to the reduction of costs for implementing of the fitosanitty tests, importants and essentials for the control of viruses prevailing in the national potato culture.

ASSUNTO(S)

anticorpo policlonal pepymv pvy pvx fitossanity control pepymv controle fitossanitario pvy pvx policlonal antibody

ACESSO AO ARTIGO

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