Polyclonal Antibodies
Mostrando 13-24 de 1669 artigos, teses e dissertações.
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13. Anti-Trimeresurus albolabris venom IgY antibodies: preparation, purification and neutralization efficacy
Abstract Background Snakebite incidence in southwestern China is mainly attributed to one of the several venomous snakes found in the country, the white-lipped green pit viper Trimeresurus albolabris. Since antivenom produced from horses may cause numerous clinical side effects, the present study was conducted aiming to develop an alternative antivenom ant
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 22/09/2016
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14. Mycobacterium paratuberculosis detection in cow's milk in Argentina by immunomagnetic separation-PCR
Abstract The aim of this study was to standardize a diagnosis procedure to detect Mycobacterium avium subsp. paratuberculosis (Map) DNA in raw cow milk samples under field conditions. A procedure that combines both immunomagnetic separation and IS900 -PCR detection (IMS-IS1 PCR) was employed on milk samples from 265 lactating Holstein cows from Map infected
Braz. J. Microbiol.. Publicado em: 2016-06
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15. Syphilis associated with paretic neurosyphilis mimicking Reiter’s syndrome in HIV-infected patients
AbstractHIV/syphilis co-infection is common because both conditions affect similar risk groups. HIV interferes with the natural history of syphilis, which often has atypical clinical features and nervous system involvement in the early stage of disease. We report the case of an HIV-positive patient with secondary syphilis, scaling palmoplantar keratoderma, s
An. Bras. Dermatol.. Publicado em: 2015-06
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16. Identification of Leptospira serovars by RFLP of the RNA polymerase beta subunit gene (rpoB)
Leptospires are usually classified by methods based on DNA-DNA hybridization and the conventional cross-agglutination absorption test, which uses polyclonal antibodies against lipopolysaccharides. In this study, the amplification of the rpoB gene, which encodes the beta-subunit of RNA polymerase, was used as an alternative tool to identify Leptospira. DNA ex
Braz. J. Microbiol.. Publicado em: 2015-06
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17. CORAL SNAKE ANTIVENOM PRODUCED IN CHICKENS (Gallus domesticus)
The production of anti-snake venom from large mammal's blood has been found to be low-yielding and arduous, consequently, antivenom immunoglobulins for treatment are achieved regularly as polyvalent serum. We have standardized an undemanding technique for making purified immunoglobulin IgY antivenom consisting of polyclonal antibodies against coral snake ven
Rev. Inst. Med. trop. S. Paulo. Publicado em: 2014-01
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18. Current management issues of immediate postoperative care in pediatric kidney transplantation
The number of pediatric kidney transplants has been increasing in many centers worldwide, as the procedure provides long-lasting and favorable outcomes; however, few papers have addressed the immediate postoperative care of this unique population. Herein, we describe the management of these patients in the early postoperative phase. After the surgical proced
Clinics. Publicado em: 2014-01
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19. Polyclonal antibodies to the putative coat protein of Citrus leprosis virus C expressed in Escherichia coli: production and use in immunodiagnosis
This work reports the in vitro expression of Citrus leprosis virus C (CiLV-C) putative coat protein (p29) and the production of a polyclonal antibody to be used as a tool for serological diagnosis of citrus leprosis. The ORF2/RNA1, corresponding to p29, was cloned in pET28a and transformed into Escherichia coli cells (BL21). Expression of p29 was induced in
Trop. plant pathol.. Publicado em: 14/05/2013
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20. Produção e eficácia de anticorpos gerados contra glicolipídios de Paracoccidioides brasiliensis / Production and efficacy of antibodies generated against glycolipids of P. brasiliensis
Paracoccidioidomycosis (PCM) is a systemic fungal infection most prevalent in Latin America, whose etiologic agent, Paracoccidioides brasiliensis, is a yeast with a complex cell wall structure consisting of proteins, glycoproteins, polysaccharides, lipids, and melanin polypeptides that gather physical, chemical and biological conditions to serve as antigens.
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 10/10/2012
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21. Desenvolvimento de imunossensores piezelétrico e eletroquímico para detecção de amostras de interesse agronômico e de saúde / Desenvolvimento de imunossensores piezelétrico e eletroquímico para detecção de amostras de interesse agronômico e de saúde / Development of piezoelectric and electrochemical immunosensors to detect samples of agronomic interest and public health / Development of piezoelectric and electrochemical immunosensors to detect samples of agronomic interest and public health
This work shows development of immunosensors for agronomic interest and public heath. The first was a piezoeletric immunosensor to detect a cysteine peptidase, a potencial biomarker for Xanthomonas citri subsp. citri that cause a citrus Canker. Another was electrochemical immunosensor based on gold nanoparticles as a label and magnetic beads as capture phase
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 23/07/2012
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22. Estudo da interação da metaloprotease BmooMPα-I, isolada da peçonha de Bothrops moojeni (HOGE, 1965; Viperidae),com a citocina pró-inflamatória TNF-α.
A inflamação é um processo essencial para manutenção da homeostase, envolvendo eventos vasculares, migração e ativação de leucócitos. No entanto, suas manifestações exacerbadas, geralmente mediadas pelo aumento do nível de citocinas pró-inflamatórias como o fator de necrose tumoral (TNF-α), podem resultar em doenças crônicas, como artri
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 29/06/2012
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23. Produção e caracterização da porção Fab do anticorpo anti-digoxina utilizando a tecnologia de phage display. / Production and characterization of the Fab portion of anti-digoxin antibody by phage display technology.
Digoxin is a pharmaceutical used in the control of cardiac dysfunction. Its therapeutic window is very narrow. To counteract the toxic effect, polyclonal anti-digoxin Fab fragments are commercially available. Our goal was to obtain variants of monoclonal anti-digoxin Fab fragments by phage display technology, which allows the generation of high affinity and
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 27/03/2012
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24. Renal macrophage infiltration is associated with a poor outcome in IgA nephropathy
OBJECTIVES: The objectives of our study were as follows: 1) to analyze the prognostic value of macrophage infiltration in primary IgA nephropathy (IgAN) and 2) to study the relationship between macrophages and other factors associated with the development of renal fibrosis, including mast cells, TGF-β1, α-SMA and NF-kB. METHODS: We analyzed 62 patients who
Clinics. Publicado em: 2012-07