Polyclonal Antibodies
Mostrando 25-36 de 1669 artigos, teses e dissertações.
-
25. Growth inhibition of Staphylococcus aureus and escherichia coli strains by neutralizing IgY antibodies from ostrich egg yolk
Ostrich raising around the world have some key factors and farming profit depend largely on information and ability of farmers to rear these animals. Non fertilized eggs from ostriches are discharged in the reproduction season. Staphylococcus aureus and Escherichia coli are microorganisms involved in animal and human diseases. In order to optimize the use of
Brazilian Journal of Microbiology. Publicado em: 2012-06
-
26. Increased immunohistochemical expression of YKL-40 in the spleen of patients with portal hypertension
YKL-40 has been identified as a growth factor in connective tissue cells and also a migration factor in vascular smooth muscle cells. To a large extent, the increase of serum YKL-40 is attributed to liver fibrosis and asthma. However, the relationship of the expression and clinical/prognostic significance of YKL-40 to the splenomegaly of patients with portal
Brazilian Journal of Medical and Biological Research. Publicado em: 2012-03
-
27. Theobroma cacao cystatins impair Moniliophthora perniciosa mycelial growth and are involved in postponing cell death symptoms.
Three cystatin open reading frames named TcCys1, TcCys2 and TcCys3 were identified in cDNA libraries from compatible interactions between Theobroma cacao (cacao) and Moniliophthora perniciosa. In addition, an ORF named TcCys4 was identified in the cDNA library of the incompatible interaction. The cDNAs encoded conceptual proteins with 209, 127, 124, and 205
Planta. Publicado em: 2011
-
28. Detection of three Allexivirus species infecting garlic in Brazil.
Garlic viruses often occur in mixed infections under field conditions. In this study, garlic samples collected in three geographical areas of Brazil were tested by Dot-ELISA for the detection of allexiviruses using monoclonal specific antibodies to detect Garlic virus A (GarV-A), Garlic virus B (GarV-B), Garlic virus C (GarV-C) and a polyclonal antiserum abl
Pesquisa Agropecuária Brasileira. Publicado em: 2011
-
29. Standardization of enzyme immunoassay (ELISA) for serology, detection and quantification of porcine circovirus type 2 / Padronização de ensaios imunoenzimáticos (ELISA) para sorologia, detecção e quantificação do circovírus suíno 2
Porcine circovirus 2 (PCV-2) is the major causative agent of postweaning multisystemic wasting syndrome (PMWS) and is associated with different syndromes that affect the swine. The term PCVAD (Porcine circovirus associated diseases) was introduced in 2006 to gather many diseases. Since serological studies are essential for monitoring the virus, the aim of th
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 07/05/2010
-
30. Expression and purification of the immunogenically active fragment B of the Park Williams 8 Corynebacterium diphtheriae strain toxin
The construction of a hexahistidine-tagged version of the B fragment of diphtheria toxin (DTB) represents an important step in the study of the biological properties of DTB because it will permit the production of pure recombinant DTB (rDTB) in less time and with higher yields than currently available. In the present study, the genomic DNA of the Corynebacte
Brazilian Journal of Medical and Biological Research. Publicado em: 2010-05
-
31. Desenvolvimento das barras imunosorventes de agitação e avaliação das técnicas extração sortiva em barra de agitação, microextração em sorvente empacotado e cromatografia líquida para análise de antidepressivos em amostras de plasma / Development of immunosorbent stir bars and evaluation of stir bar sorptive extraction, microextraction by packed sorbent and liquid chromatography for the analysis of antidepressants in plasma samples
In this work, polyclonal and monoclonal anti-fluoxetine antibodies were developed in rabbits and mice by immunization with fluoxetine-bovine albumin conjugate, respectively. The developed antibodies were characterized on the basis of the specificity against the drug by ELISA (enzyme linked immunosorbent assay) and, subsequently they were purified by labmade
Publicado em: 2010
-
32. Expressão do fator estimulador de colônia de granulócito humano recombinante (rhG-CSF) em Escherichia coli. / Expression of recombinant human colony stimulating factor (rhG-CSF) in Escherichia coli.
The recombinant human granulocyte colony stimulating factor (rhG-CSF) is a non-glycosylated protein with 175 amino acids. This factor plays an important role in hematopoietic cell proliferation and has been widely used for treating neutropenia. The purpose of this work is to construct two expression systems in E. coli; a system for obtaining rhG-CSF in the c
Publicado em: 2010
-
33. Modificação pós-traducional pendente de SUMO em Schistosoma mansoni: padrão de expressão diferencial durante a transição de cercária a esquistossômulo
Attachment of SUMO to target proteins occur through by similar mechanism to ubiquitination, with sequential action of three enzymes: E1 activating (Aos1-Uba2), E2 conjugation (Ubc9) and E3 ligases (PIAS, Polycomb2 and RanBP2). SUMO is synthesized as inactive precursors that require processing by specific proteases (SENPs). SUMO attachment is a reversible and
Publicado em: 2010
-
34. Anticorpos anti-intimina: análise da reatividade dos anticorpos policlonal e monoclonal, clonagem e expressão do fragmento variável de cadeia simples (scFv) do anticorpo monoclonal / Anti-intimin antibodies: polyclonal and monoclonal reactivity analyzes, cloning and expression of single chain fragment variable (scFv) of monoclonal antibodies
Intimina é o principal fator de virulência envolvido na patogênese de Escherichia coli enteropatogênica (EPEC) e de Escherichia coli enterohemorrágica (EHEC). A detecção de EHEC e EPEC típica ou atípica é de fundamental importância na definição da conduta terapêutica das infecções promovidas por E. coli, que ainda são a principal causa de di
Publicado em: 2010
-
35. Quantification of natural populations of Gluconacetobacter diazotrophicus and Herbaspirillum spp. In sugar cane (Saccharum spp.) Using differente polyclonal antibodies
The species Gluconacetobacterdiazotrophicus, Herbaspirillum seropedicae and H. rubrisubalbicans are endophytic N2-fixing [diazotrophic] bacteria which colonise not only roots, but also the aerial tissue of sugar cane. However, the technique most commonly used to quantify the populations of these microbes in plants is by culturing serial dilutions of macerate
Brazilian Journal of Microbiology. Publicado em: 2009-12
-
36. Quasispecies detection in human respiratory syncytial virus (HRSV) samples in absence and presence of polyclonal serum / Detecção de quasispecies em amostras de vírus respiratório sincicial humano (HSRV) na ausência e na presença de soros policlonais
Human respiratory syncytial virus (HRSV) is one of the most important clinical respiratory pathogens, since 64 millions children in the world are infected by this agent every year. Host immunity and viral genetic variability are important factors to a vaccine development, besides quasispecies presence in the viral population. In this work, HRSV quasispecies
Publicado em: 2009