Insect Cells Culture
Mostrando 1-12 de 95 artigos, teses e dissertações.
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1. Inhibition of Brazilian ZIKV strain replication in primary human placental chorionic cells and cervical cells treated with nitazoxanide
ABSTRACT Zika virus (ZIKV) infection during pregnancy is associated with a congenital syndrome. Although the virus can be detected in human placental tissue and sexual transmission has been verified, it is not clear how the virus reaches the fetus. Despite the emerging severity caused by ZIKV infection, no specific prophylactic and/or therapeutic treatment i
Braz J Infect Dis. Publicado em: 2020-12
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2. Correlation of meta 1 expression with culture stage, cell morphology and infectivity in Leishmania (Leishmania) amazonensis promastigotes
The parasitic protozoan Leishmania (Leishmania) amazonensis alternates between mammalian and insect hosts. In the insect host, the parasites proliferate as procyclic promastigotes andthen differentiate into metacyclic infective forms. The meta 1 gene is preferentially expressed during metacyclogenesis. Meta 1 expression profile determination along parasite g
Memórias do Instituto Oswaldo Cruz. Publicado em: 2011-03
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3. Cultura de células de Drosophila melanogaster (S2) em processo contínuo. / Culture of Drosophila melagogaster cells (S2) in continuous culture.
As células de Drosophila melanogaster (S2) têm sido utilizadas como sistemas de expressão de proteínas recombinantes. Neste trabalho foi utilizada uma linhagem S2 geneticamente modificada com vetores de expressão para a produção da glicoproteína do vírus da raiva (GPV). O principal objetivo deste trabalho foi avaliar o comportamento destas células
Publicado em: 2010
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4. Efeito da adição de colesterol e ecdisona na produção in vitro do baculovírus spodoptera frugiperda MNPV
Dentre as pragas que atacam a cultura do milho no Brasil, destaca-se a Spodoptera frugiperda (J. E. Smith, 1797) (Lepidoptera: Noctuidae), conhecida no estádio larval como lagarta-do-cartucho, considerada a praga chave da cultura, alimentando-se da planta em todas as suas fases de crescimento, principalmente dos cartuchos de plantas jovens. Para seu control
Publicado em: 2010
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5. Controle hormonal da defesa à herbivoria em tomateiro / Hormonal control of herbivory defense in tomato
Even though the cultivation of tomato has a high economical importance, this culture is classified as being of elevated risk because of it common infestation by pests and diseases. This problem makes this culture greatly dependent of the application of several agrochemicals, which leads to increase in cost of production, environmental damages and also facili
Publicado em: 2009
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6. Caracterização molecular de mutantes gerados pela passagem serial do baculovírus Anticarsia gemmatalis MNPV em cultura de células
Baculovirus are insect viruses with great application potential for biological control programs due mainly to their ecological appeal, once they are highly specific to the host insect and do not present toxicity to man and to the environment. Their production has been made basically by their multiplication in host insects. Nevertheless, baculovirus productio
Publicado em: 2008
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7. Efeito da temperatura na otimização da produção de glicoproteína do vírus da raiva em cultivos em suspensão de células recombinantes de Drosophila melanogaster S2
The increment of the expression of complex correctly processed recombinant proteins, like the ones produced by animal cells, is essential for the development of an efficient large scale bioprocess. The manipulation of culture conditions plays a very important role in the controlled and optimized expression of these proteins in bioreactors. The response of ma
Publicado em: 2008
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8. Análise citopatológica de células de inseto infectadas com baculovírus mutantes
Advances in cell culture techniques allowed the isolation and precise characterization of baculoviruses mutants. The majority of these mutants is derived from the virus AcMNPV, mainly due to its facility of propagation and stability in cell culture. During the purification routine of a recombinant baculovirus, two mutants derived from AcMNPV, vSynlitx1B12P (
Publicado em: 2007
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9. Expressão da proteína do envelope do vírus da Febre Amarela em células de inseto
Yellow fever is an haemorrhagic disease caused by a virus that belongs to the genus Flavivirus (Flaviviridae family) and is transmitted by mosquitoes. It is a positive- sense, single-stranded, enveloped RNA virus, and its genome consists of 10,862 nucleotides coding for a single ORF of 10,233 nucleotides. This ORF encodes three structural proteins (capsid, p
Publicado em: 2007
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10. EFEITOS DE Beauveria bassiana (Bals.) Vuill. E Metarhizium anisopliae (Metsch.) Sorok. SOBRE PARÂMETROS BIOLÓGICOS E FISIOLÓGICOS DE Diatraea saccharalis F. (LEPIDOPTERA: CRAMBIDAE) / EFFECTS OF Beauveria bassiana (Bals.) Vuill. AND Metarhizium anisopliae (Metsch.) Sorok. ON BIOLOGICAL AND PHYSIOLOGICAL PARAMETERS OF Diatraea saccharalis F. (LEPIDOPTERA: CRAMBIDAE)
The sugarcane borer Diatraea saccharalis F. is considered one of the main pests in America, being responsible for direct and indirect damages to this culture. The use of entomophatogenic fungi for the control of pests has been a lot intensified in the last decades, and the fungi Beauveria bassiana (Bals.) Vuill. and Metarhizim anisopliae (Metch.) Sorok were
Publicado em: 2006
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11. Efeitos do inseticida organofosforado metil paration (Folisuper 600 BR) sobre biomarcadores do estresse oxidante no teleósteo de água doce matrinxã Brycon cephalus (GUNTER, 1869)
Methyl parathion (MP) is an organophosphorus insecticide worldwide used in agriculture and aquaculture due to its high activity against a broad spectrum of insect pests. It is employed as pesticide in agriculture and in food storage shelters, as well as in fish culture tanks to kill aquatic larval stages of insects. The effect of exposure to 2 ppm of a comme
Publicado em: 2006
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12. Apoptotic mimicry in Leishmania spp.: role in host/parasite interaction. / Mimetismo apoptótico em Leishmania spp.: papel na interação parasita/hospedeiro
Promastigotes of Leishmania are found in the midgut environment of the vector insect and can be grown in some culture medium. In culture the genetically regulated differentiation from procyclic to infective metacyclic can be observed. Amastigotes are intracellular parasites and which multiply inside phagolysosomes of macrophages. Metacyclics and amastigotes
Publicado em: 2004