Correlation of meta 1 expression with culture stage, cell morphology and infectivity in Leishmania (Leishmania) amazonensis promastigotes
AUTOR(ES)
Santos, Marcos Gonzaga dos, Silva, Maria Fernanda Laranjeira da, Zampieri, Ricardo Andrade, Lafraia, Rafaella Marino, Floeter-Winter, Lucile Maria
FONTE
Memórias do Instituto Oswaldo Cruz
DATA DE PUBLICAÇÃO
2011-03
RESUMO
The parasitic protozoan Leishmania (Leishmania) amazonensis alternates between mammalian and insect hosts. In the insect host, the parasites proliferate as procyclic promastigotes andthen differentiate into metacyclic infective forms. The meta 1 gene is preferentially expressed during metacyclogenesis. Meta 1 expression profile determination along parasite growth curves revealed that the meta 1 mRNA level peaked at the early stationary phase then decreased to an intermediate level. No correlation was observed between meta 1 expression and infectivity. Conversely, infectivity correlated with the increase of apoptotic cells in the late stationary phase.
Documentos Relacionados
- Role of Leishmania (Leishmania) amazonensis amastigote glycosphingolipids in macrophage infectivity
- Subcellular localization of an intracellular serine protease of 68 kDa in Leishmania (Leishmania) amazonensis promastigotes
- Characterization of the Leishmania (Leishmania) amazonensis genomic region containing the META 1 gene.
- Miltefosine induces programmed cell death in Leishmania amazonensis promastigotes
- Cell migration induced by Leishmania (Leishmania) amazonensis, Leishmania (Leishmania) major and Leishmania (Viannia) braziliensis into the peritoneal cavity of BALB/c mice