Hemagglutinating Activity
Mostrando 13-24 de 187 artigos, teses e dissertações.
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13. The alga Bryothamnion seaforthii contains carbohydrates with antinociceptive activity
Bryothamnion seaforthii, a red alga common to the Northeastern coast of Brazil, was used to prepare the protein fraction F0/60 by ammonium sulfate precipitation. The chromatography of F0/60 on DEAE-Sephadel column resulted in two lectin fractions, PI and PII, which have antinociceptive properties in rodents. We determined the antinociceptive activity of the
Brazilian Journal of Medical and Biological Research. Publicado em: 2004-07
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14. PurificaÃÃo e caracterizaÃÃo da lectina da vagem de Caesalpinia ferrea (cfepl): aplicaÃÃo biolÃgica
Lectins are proteins that bind specifically and reversibly to carbohydrates. Caesalpinia ferrea is a leguminous tree widely distributed in Brazil used in popular medicine. The aim of this work was the purification and characterization of C. ferrea pod lectin (CfePL). Pod extract (E) in 0.15 M NaCl was partially purified on activated charcoal followed by ammo
Publicado em: 2004
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15. Isolamento, caracterizaÃÃo parcial e imobilizaÃÃo da lectina de pericarpo de Punica granatum l. em dacron magnetizado
Nature is a source of active substances and pericarp of Punica granatum Linn. (romÃ, pomegranate) has been used in popular medicine. Hemagglutinating proteins (lectins) interact selectively and reversibly with carbohydrates and glycoconjugates. The aim of this work was the isolation of P. granatum pericarp lectin (PgPeL) and its immobilization to produce an
Publicado em: 2002
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16. Isolamento, caracterizaÃÃo parcial e atividades biolÃgicas da lectina de entrecasca de aroeira (Schinus terebinthifolius Raddi.)
Bark infusions of Schinus terebinthifolius Raddi., Anacardiaceae family (aroeira), have been commonly used in popular medicine. Lectins constitute a heterogeneous protein group capable of recognizing and reversibly bind to carbohydrates. The aim of this work was to isolate a bark lectin from S. terebinthifolius and to evaluate its biological properties. Extr
Publicado em: 2002
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17. Desnaturação e reenovelamento da frutalina, uma lectina ligante de D galactose / Folding and unfolding of frutalin lectin
Our current understanding of the protein folding mechanism is the result of intense study employing biophysical, biochemical and theoretical methods. "In vitro", the initial state of the protein in this puzzle is its unfolded form. In the present work we have studied the refolding, after thermal denaturation, of the glycoprotein frutalin, a member of the lec
Publicado em: 1998
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18. Hemagglutinating activity of lipopolysaccharides from subgingival plaque bacteria.
Lipopolysaccharide (LPS) preparations from the Bacteroides species B. endodontalis, B. intermedius, B. denticola, and B. melaninogenicus and from Fusobacterium nucleatum, Haemophilus actinomycetemcomitans, Capnocytophaga gingivalis, and Eikenella corrodens directly agglutinated erythrocytes of some kinds of animals. LPSs from the Bacteroides species B. gingi
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19. Hemagglutination activity of Treponema denticola grown in serum-free medium in continuous culture.
Hemagglutination by different Treponema denticola strains was observed for erythrocytes of human, horse, bovine, and rabbit origin. The growth of T. denticola ATCC 33520 in serum-free medium in continuous culture enabled us to study the hemagglutinating activity of freshly harvested spirochetes of a defined physiological status. The hemagglutinating activity
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20. Point mutations in the S protein connect the sialic acid binding activity with the enteropathogenicity of transmissible gastroenteritis coronavirus.
Enteropathogenic transmissible gastroenteritis virus (TGEV), a porcine coronavirus, is able to agglutinate erythrocytes because of sialic acid binding activity. Competitive inhibitors that may mask the sialic acid binding activity can be inactivated by sialidase treatment of virions. Here, we show that TGEV virions with efficient hemagglutinating activity we
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21. Purification and properties of hemagglutinin from culture supernatant of Bacteroides gingivalis.
The hemagglutinating factor (hemagglutinin) of Bacteroides gingivalis was prepared from the supernatant of a 5-day diffusate broth culture by ammonium sulfate precipitation and column chromatography with a hydrophobic column of Phenyl-Sepharose CL-4B, DEAE-Sephadex A-50, and Sephadex G-100 gel filtration. The hemagglutinating activity of the preparation was
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22. Hemagglutination, autoagglutination and pathogenicity of Moraxella bovis strains.
Three isolates of Moraxella bovis, recovered from cattle with signs of infectious bovine keratoconjunctivitis, were tested for autoagglutinating activity, hemagglutinating activity and pathogenicity in young calves. Only the autoagglutinating and hemagglutinating isolates were pathogenic in calves. Treatment of the pathogenic isolates with magnesium chloride
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23. A specific cell surface antigen of Streptococcus gordonii is associated with bacterial hemagglutination and adhesion to alpha2-3-linked sialic acid-containing receptors.
A Ca2+-independent lectin activity for alpha2-3-linked sialic acid-containing receptors is associated with Streptococcus gordonii DL1 (Challis) but not with a spontaneous mutant, strain D102, that specifically lacks hemagglutinating activity. Comparison of crossed-immunoelectrophoresis patterns of parent and mutant sonicated cell extracts identified a unique
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24. In vivo adherence and colonization of Vibrio cholerae strains that differ in hemagglutinating activity and motility.
A scanning electron microscopic study was carried out to compare the in vivo pathogenicity of two strains of Vibrio cholerae in an adult rabbit ligated-gut test model. V. cholerae C5 (serotype Ogawa, biotype El Tor), a motile strain possessing hemagglutinating activity in vitro, and C21 (serotype Ogawa, classical biotype), a nonmotile strain possessing no he