Isolamento, caracterizaÃÃo parcial e atividades biolÃgicas da lectina de entrecasca de aroeira (Schinus terebinthifolius Raddi.)

AUTOR(ES)

Ana Mary Alves Viana

DATA DE PUBLICAÇÃO

2002

RESUMO

Bark infusions of Schinus terebinthifolius Raddi., Anacardiaceae family (aroeira), have been commonly used in popular medicine. Lectins constitute a heterogeneous protein group capable of recognizing and reversibly bind to carbohydrates. The aim of this work was to isolate a bark lectin from S. terebinthifolius and to evaluate its biological properties. Extract was obtained from bark powder and submitted to hemagglutinating activity (HA) assays using rabbit and human erythrocytes; heated extract (100Â C by 30 min) was also used. Proteins of the extract were precipitated with ammonium sulphate and a 40% supernatant fraction (SF40) was chromatographed on a chitin column; 0.15 M sodium borate buffer (pH 7.4) and 1 M NaCl were used at the elution steps. Inhibition assay of SF40 activity was performed with carbohydrates (fructose, fucose, galactose, glucose, maltose, mannose, N-acetylglucosamine, raffinose, rhamnose and xylose), as well as glycoproteins (casein, fetuin, ovalbumin and thyroglobulin). S. terebinthifolius bark lectin (SteBL) was studied with regard to their effects on release of hydrogen peroxide or nitric oxide by macrophage cells and lymphocyte proliferation. SteBL agglutinated erythrocytes at different degrees; the highest specific HA was detected with human AB cells. The lectin activity was stimulated after heating and inhibition was obtained with fucose, galactose, Nacetylglucosamine, raffinose and glycoproteins. The chromatographic profile showed protein peaks eluted with sodium borate and 1 M NaCl; only the latter peak promoted hemagglutination. SteBL had nitric oxide-producing properties, induced release of H2O2 by macrophages and showed low mitogenic activity. SteBL, a new bark lectin, has perspectives of biotechnological applications

ASSUNTO(S)

atividades biolÃgicas - caracterizaÃÃo parcial -isolamento bioquimica aroeira (schinus terebinthifolius raddi.) - lectina de entrecasca

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