Isolamento, caracterizaÃÃo parcial e imobilizaÃÃo da lectina de pericarpo de Punica granatum l. em dacron magnetizado

AUTOR(ES)
DATA DE PUBLICAÇÃO

2002

RESUMO

Nature is a source of active substances and pericarp of Punica granatum Linn. (romÃ, pomegranate) has been used in popular medicine. Hemagglutinating proteins (lectins) interact selectively and reversibly with carbohydrates and glycoconjugates. The aim of this work was the isolation of P. granatum pericarp lectin (PgPeL) and its immobilization to produce an affinity matrix. Extract of pericarp was submitted to ammonium sulphate precipitation and the fractions obtained were evaluated with regard to its effects on erythrocyte agglutination (rabbit and human types). The most active 60% supernatant fraction (SF60) was used to evaluate lectin specificity (carbohydrates and glycoproteins), protein thermic stability (30 to 100ÂC, 30 min or 100ÂC, 60 min) and the effect of ions (Ca2+ and Mg2+) or pH (6.5 to 8.0 values) on hemagglutinating activity. PgPeL obtained after chromatography of SF60 on Sepharose CL-6B column (purification of 10 folds) was immobilized on ferromagnetic Dacron (FMD); PgPeL-FMD was applied to fetuin and ovalbumin isolation. SF60 agglutinated all human erythrocytes (titer of 512-1); but the best result was obtained with rabbit cells (titer of 2,048-1). PgPeL activity was not stimulated by divalent ions and was inhibited by fructose, mannose, casein, fetuin, ovalbumin, as well as thyroglobulin. The highest lectin activity (pH 7.5) was totally lost at pH 8.0. PgPel was active (titer of 4-1) after heating to 100ÂC. Immobilized PgPeL (87%) was able to bind fetuin (160 mg), but ovalbumin was not retained. PgPeL-FMD can be used to glycoprotein purification in a very fast and simple method

ASSUNTO(S)

lectina punica granatum linn dacron magnetizado bioquimica

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