Glycosyl Hydrolases
Mostrando 1-12 de 63 artigos, teses e dissertações.
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1. A broad pH range and processive chitinase from a metagenome library
Chitinases are hydrolases that degrade chitin, a polymer of N-acetylglucosamine linked β(1-4) present in the exoskeleton of crustaceans, insects, nematodes and fungal cell walls. A metagenome fosmid library from a wastewater-contaminated soil was functionally screened for chitinase activity leading to the isolation and identification of a chitinase gene nam
Braz J Med Biol Res. Publicado em: 05/01/2017
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2. ETHANOL PRECIPITATION OF GLYCOSYL HYDROLASES PRODUCED BY Trichoderma harzianum P49P11
AbstractThis study aimed to concentrate glycosyl hydrolases produced by Trichoderma harzianum P49P11 by ethanol precipitation. The variables tested besides ethanol concentration were temperature and pH. The precipitation with 90% (v/v) ethanol at pH 5.0 recovered more than 98% of the xylanase activity, regard less of the temperature (5.0, 15.0, or 25.0 °C).
Braz. J. Chem. Eng.. Publicado em: 2015-06
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3. Cloning of a novel xylanase gene from a newly isolated Fusarium sp. Q7-31 and its expression in Escherichia coli
A strain of Q7-31 was isolated from Qinghai-Tibet Plateau and was identified as Fusarium sp. based on its morphological characteristics and ITS rDNA gene sequence analysis. It has the highest capacity of degrading cell wall activity compared with other 11 strains. To do research on its xylanase activity of Fusarium sp. Q7-31 while the degrading the rice cell
Brazilian Journal of Microbiology. Publicado em: 2012-03
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4. Construção de biblioteca metagenômica de DNA microbiano do solo amazônico e prospecção de enzimas de interesse biotecnológico
Problemas relacionados a diminuicao dos estoques de combustiveis fosseis, politicos e ambientais tem levado a busca de alternativas renovaveis. A biomassa vegetal e abundante e barata, sendo candidata ideal para producao de biocombustiveis; entanto, o material lignocelulosico presente na parede celular das plantas possui estrutura complexa e atualmente nao e
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/06/2011
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5. Caracterização bioquímica e molecular da ß-Galactosidade durante a maturação de frutos de coffea arabica
ß-galactosidases are a class of glycosyl-hydrolases that act on the plant cell primary walls, hydrolyzing ß-D-galactose at the nonreducing ends of ß-D-galactosides present in several biological molecules. Initially a characterization of the monosaccharides present in the primary wall of the pericarp and endosperm of Coffea arabica fruits at different ripe
Publicado em: 2011
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6. Analise filogenetica das enzimas hidroliticas de Xiloglucano no reino Viridiplantae e construção de bibliotecas de cDNA de Jatoba (Hymenaea courbaril) / Phylogenetic analysis of Xyloglucan s hydrolytic enzymes in the Viridiplantae kingdom and construction of cDNA libraries from Jatoba (Hymenaea courbaril)
Introdução: Os xiloglucanos são os polímeros de açúcar mais abundantes na hemicelulose da maioria das espécies de plantas terrestres, em especial nas eudicotiledôneas. Possuem papel estrutural na parede celular vegetal, interagindo com os filamentos de celulose, e podem ser utilizados como reserva em sementes de várias espécies de eudicotiledôneas
Publicado em: 2008
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7. Structural and functional studies of two glycosyl hydrolases : the putaitve cellulase XF0810 of Xylella fastidiosa and the digestive lysozyme 1 of Musca domestica / Estudos estruturais e funcionais de duas glicosideo hidrolases : a celulase putativa XF0810 de Xylella fastidiosa e a lisozima digestiva 1 de Musca domestica
Glicosídeo hidrolases (EC 3.2.1.-) são enzimas que hidrolisam ligações glicosídicas. Neste trabalho realizamos estudos funcionais e estruturais de duas glicosídeo hidrolases: a celulase XF0810 de Xylella fastidiosa e a lisozima digestiva 1 de Musca domestica (MdL1). A celulase XF0810 está anotada no genoma da X. fastidiosa como membro da família 5 da
Publicado em: 2007
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8. Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases.
The regions surrounding the catalytic amino acids previously identified in a few "retaining" O-glycosyl hydrolases (EC 3.2.1) have been analyzed by hydrophobic cluster analysis and have been used to define sequence motifs. These motifs have been found in more than 150 glycosyl hydrolase sequences representing at least eight established protein families that
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9. Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases
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10. Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases.
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11. Intra- and Extracellular β-Galactosidases from Bifidobacterium bifidum and B. infantis: Molecular Cloning, Heterologous Expression, and Comparative Characterization
Three β-galactosidase genes from Bifidobacterium bifidum DSM20215 and one β-galactosidase gene from Bifidobacterium infantis DSM20088 were isolated and characterized. The three B. bifidum β-galactosidases exhibited a low degree of amino acid sequence similarity to each other and to previously published β-galactosidases classified as family 2 glycosyl hyd
American Society for Microbiology.
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12. Biochemical and Phylogenetic Analyses of a Cold-Active β-Galactosidase from the Lactic Acid Bacterium Carnobacterium piscicola BA
We are investigating glycosyl hydrolases from new psychrophilic isolates to examine the adaptations of enzymes to low temperatures. A β-galactosidase from isolate BA, which we have classified as a strain of the lactic acid bacterium Carnobacterium piscicola, was capable of hydrolyzing the chromogen 5-bromo-4-chloro-3-indolyl β-d-galactopyranoside (X-Gal) a
American Society for Microbiology.