Glucosyltransferase
Mostrando 13-24 de 304 artigos, teses e dissertações.
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13. Produção de isomaltulose a partir da transformação enzimatica da sacarose, utilizando-se Erwinia sp D12 imobilizada com alginato de calcio
Isomaltulose (6-0-a-D-glucopyranosyl-D-fiuctofuranose) lS a reducing disaccharide, structural isomer of sucrose, naturally found in honey and sugar cane juice. Due to its low cariogenic potential, isomaltulose is commerciallyused to produce candies and chewing gums. Isomaltulose is also used to produce isomalt, a sugar alcohol characterized by its low calori
Publicado em: 2002
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14. Polimorfismo genetico de Streptococcus mutans isolados da cavidade oral de individuos com lesões de carie coronaria e radicular
Dental root caries has been considered a very significant oral health problem in elderly and adults. Studies have suggested a common etiology for root and coronal caries, involving mutans streptococci. The aim of this study was to evaluate the colonization profile and clonal distribution of Streptococcus mutans isolated from oral cavity, presenting caronal a
Publicado em: 2002
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15. Avaliação do potencial anti-carie e anti-placa de propolis de Apis mellifera selecionadas de duas regiões do Brasil
The aim of the present study was to evaluate the anti-caries and anti-plaque potential of Apis. mellifera propolis collected from two regions of Brazil. The ethanolic extract of propolis (EEP) was prepared from crude propolis samples collected in Southeastern Brazil and Southern Brazil. The EEPs were screened and selected by two parameters: 1) chemical compo
Publicado em: 2000
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16. Regulation and extracellular glucosyltransferase production and the relationship between extracellular and cell-associated activities in Streptococcus mutans.
The regulation of extracellular glucosyltransferase production in Streptococcus mutans GS-5 has been studied using a chemically defined medium. Most of the glucosyltransferase activity produced by cells grown in the chemically defined medium was extracellular, in contrast with the distribution between cell-associated and extracellular glucosyltransferase act
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17. Production of Extracellular and Cell-Associated Glucosyltransferase Activity by Streptococcus mutans During Growth on Various Carbon Sources
The production of extracellular and cell-associated glucosyltransferase activity by Streptococcus mutans strain GS-5 was examined during growth on various carbon sources in a chemically defined medium. S. mutans cells produced glucosyltransferase activity only during logarithmic growth when glucose, fructose, mannitol, or sorbitol was the sole carbon source.
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18. Effect of Tween 80 on glucosyltransferase production in Streptococcus mutans.
Glucan production from sucrose by Streptococcus mutans OMZ 176 was stimulated approximately threefold in the presence of 0.1% Tween 80. When OMZ 176 was grown in a medium containing glucose, the glucosyltransferase level in the medium was also increased about fivefold in the presence of 0.1% Tween 80. The glucosyltransferase level increased in proportion to
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19. Nucleotide sequence of a glucosyltransferase gene from Streptococcus sobrinus MFe28.
The complete nucleotide sequence was determined for the Streptococcus sobrinus MFe28 gtfI gene, which encodes a glucosyltransferase that produces an insoluble glucan product. A single open reading frame encodes a mature glucosyltransferase protein of 1,559 amino acids (Mr, 172,983) and a signal peptide of 38 amino acids. In the C-terminal one-third of the pr
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20. Effect of oral administration of glucosyltransferase antigens on experimental dental caries.
The effect of oral administration of soluble antigen preparations containing glucosyltransferase on dental caries in hamsters was studied. Immunization was accomplished by feeding glucosyltransferase for 21 to 27 consecutive days. This immunization regimen resulted in the formation of salivary antibody, which was detected by functional inhibition of enzymati
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21. Requirements for fatty acid synthesis and a chelation-sensitive step in the production of glucosyltransferase by Streptococcus mutans.
The antibiotic cerulenin differentially inhibited the production of glucosyltransferase activity by Streptococcus mutans GS5. Cerulenin preferentially inhibited [14C]acetate incorporation into cellular lipids but did not affect protein synthesis or ribonucleic acid synthesis in the same manner. No significant intracellular accumulation of glucosyltransferase
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22. Genetic Analysis of the rgg-gtfG Junctional Region and Its Role in Streptococcus gordonii Glucosyltransferase Activity
Glucans synthesized by glucosyltransferase enzymes of oral streptococci facilitate bacterial accumulation on surfaces. The Streptococcus gordonii glucosyltransferase gene, gtfG, is positively regulated by rgg, which encodes a putative cytoplasmic protein. The gtfG promoter and ribosomal binding sequences are located within a DNA inverted repeat immediately d
American Society for Microbiology.
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23. The molecular basis for the recognition of misfolded glycoproteins by the UDP-Glc:glycoprotein glucosyltransferase.
The UDP-Glc:glycoprotein glucosyltransferase is a soluble enzyme of the endoplasmic reticulum that glucosylates protein-linked Man7-9GlcNAc2 to form the monoglucosylated derivatives. In vivo the reaction products are immediately deglucosylated by glucosidase II. The glucosyltransferase has a unique property: it glucosylates misfolded, but not native, glycopr
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24. Preparation of glucosyltransferase from Streptococcus mutans by elution from water-insoluble polysaccharide with a dissociating solvent.
Glucosyltransferase (EC 2.4.1.5) was obtained by dissociation from water-insoluble polysaccharide in the presence of 6 M guanidine-hydrochloride. Water-insoluble polysaccharide was synthesized by cell-free culture supernatants from Streptococcus mutans strain 6715. Gel filtration of the glucosyltransferase on a column of 8% agarose in phosphate buffer, follo