Avaliação do potencial anti-carie e anti-placa de propolis de Apis mellifera selecionadas de duas regiões do Brasil

AUTOR(ES)
DATA DE PUBLICAÇÃO

2000

RESUMO

The aim of the present study was to evaluate the anti-caries and anti-plaque potential of Apis. mellifera propolis collected from two regions of Brazil. The ethanolic extract of propolis (EEP) was prepared from crude propolis samples collected in Southeastern Brazil and Southern Brazil. The EEPs were screened and selected by two parameters: 1) chemical composition analysis by reversed-phase High Performance Thin Layer Chromatography and reversed-phase High Performance Liquid Chromatography~ 2) In vitro antimicrobial activity and inhibition of crude glucosyltransferase (Gtt) enzyme assays. Two samples - one from Minas Gerais state (EEP MG) and another ITom Rio Grande do Sul state (EEP RS) - were analyzed by: 1) in vitro studies - a) Antimicrobial activity against some oral pathogens by agar diffusion method, b) determination of minimum ínhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of EEP against mutans streptococci, c) inhibition of adherence of Streptococcus mutans and S. sobrinus to glass surface, d) inhibition ofwater insoluble glucan (WIG) synthesis, e) Inhibition of activity of purified Gtfs in solution and adsorbed on the surface of hydroxyapatite, t) Inhibition of adherence of S. mutans and S. sobrinus on the surface of hydroxyapatite~ 2) animal study performed by experimental dental caries model using desalivated rats~ 3) in vivo study conducted to evaluate dental plaque inhibition. Both propolis samples (EEP MG and RS) showed significant inhibitory activities on alI in vitro parameters tested when compared to control (80 % aqueous ethanol, v/v) (p<0.05). However, EEP RS demonstrated significantly better results than EEP MG (p<0.05). In animal study, only EEP RS reduced both smooth-surface and sulcal caries and the data were statistically different to the control group (p<0.05). The mouthrinse for:mulation containing EEP RS significantly reduced dental plaque formation on smooth-surface in vivo (44,7%) when compared to placebo (p<0.05). In conclusion, propolis showed anti-caries and anti¬plaque properties. However its biological effects is variable depending on geographic origin of propolis samples due to differences in the chemícal composition ofthis natural substance

ASSUNTO(S)

caries dentarias placas dentarias streptococcus mutans

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